Tissue culture method for kalanchoe daigremontiana

A technology of ground rooting and tissue culture, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as inappropriate for large-scale cultivation, inconvenient growth of adventitious buds, etc., to reduce the occurrence of diseases, The effect of increasing the rooting rate

Inactive Publication Date: 2019-05-31
刘艳涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, when the existing large-leaf cultivation method of rooting on the ground cultivates new individuals, it is necessary to select healthy adventitious buds, which is inconvenient to control the growth of differ

Method used

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Examples

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Embodiment Construction

[0031] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0032] A kind of tissue culture method of big leaves falling to the ground and taking root of the present invention, comprises the following steps:

[0033] Material acquisition: pick fresh healthy leaves of large leaves and take root, rinse the surface of the picked leaves under tap water for 20 minutes;

[0034] Aseptic treatment: Turn on the ultraviolet germicidal lamp and fan of the aseptic operation table 30 minutes before the operation, turn off the fan 10 minutes, and fully sterilize the aseptic operation table through the ultraviolet germicidal lamp. Soak the table with 75% alcohol for 15 seconds, rinse once with sterile water after soaking, then soak in 0.1% mercuric chloride solution for disinfect...

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Abstract

The invention relates to the technical field of in vitro culture, in particular to a tissue culture method for kalanchoe daigremontiana. The method can achieve mass culture, conveniently control a growth state, and provide a novel path for rapid propagation of the kalanchoe daigremontiana and reservation of germplasm resources; the method includes the following steps of (1) material acquisition; (2) aseptic processing; (3) callus induction; (4) callus differentiation; (5) adventitious bud proliferation; (6) rooting culture; (7) refining and transplanting; (8) transplanting culture.

Description

technical field [0001] The invention relates to the technical field of in vitro culture, in particular to a tissue culture method for large leaves to take root. Background technique [0002] As we all know, the big leaf rooting is a perennial succulent herb of the sedum family Kalanchoe, 50-100cm high, with solitary stems, erect, brown, and lignified bases. The leaves are opposite or whorled, the lower leaves are larger, often clasping the stem; the leaves are fleshy, triangular in shape, with irregular brownish-purple markings, and the leaf margins have coarse teeth, and seedlings with 2-4 true leaves grow from the sawtooth. Touching the ground, the roots go deep into the soil to form new pillars, so it is called rooting on the ground. Compound cymes, terminal, multi-branched, bell-shaped flowers, orange-red, flowering period from April to June. Native to the tropical regions of Madagascar, Africa, it can now be cultivated in many parts of the world. [0003] The leaves ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘艳涛胡铁欢钮力亚尹振君
Owner 刘艳涛
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