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A kind of method for artificial hybridization of Dendrobium nobile

A technology of artificial hybridization of Dendrobium nobile, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of difficult pollination of Dendrobium nobile, unfavorable development and utilization of resources, and unfavorable preservation of resources of Dendrobium nobile

Active Publication Date: 2020-12-18
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous development of Dendrobium nobile, the exploitation of wild resources is waiting to be exhausted, and the artificial cultivation products are gradually converging, which is not conducive to the preservation of Dendrobium nobile resources, and is not conducive to the further development and utilization of resources
Under natural conditions, it is difficult for Dendrobium nobile to pollinate, and the fruit setting rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The experiment site is in the nursery base of Guizhou Normal University in Guiyang City, Guizhou Province. Three-year-old Dendrobium nobile with strong growth and healthy plants is selected. Among them, Chishui Dendrobium nobile is the female parent, and Emeishan Dendrobium nobile is the male parent. , only 3 to 5 flower buds are kept on each Vitex, and the pollen of Dendrobium nobile in Mount Emei is collected according to the conventional method, and the pollen is stored in a refrigerator at 0 to 2°C. Before pollination, the pollen is taken out from the refrigerator and Cultivate at 15° C. for 2 hours and then at 25° C. for 2 hours. The ambient humidity of the cultivation is 80% to obtain cultured pollen, which is pollinated to Dendrobium nobile by conventional pollination methods.

[0027] A total of 1121 Dendrobium nobile flowers were artificially cross-pollinated, and the plants grew well after pollination, and no adverse symptoms such as leaf wilting occurred. The...

Embodiment 2

[0032] The experiment site is in the nursery base of Guizhou Normal University in Guiyang City, Guizhou Province. Three-year-old Dendrobium nobile with vigorous growth and healthy plants is selected. The Chishui Dendrobium nobile is the male parent, and the Emeishan Dendrobium nobile is the female parent. , only 3 to 5 flower buds are kept on each Vitex, and the pollen of Dendrobium nobile is collected according to the conventional method, and the pollen is stored in a refrigerator at 0 to 2°C. Before pollination, the pollen is taken out from the refrigerator and Cultivate at 14° C. for 2 hours and then at 24° C. for 2 hours at 80% ambient humidity to obtain cultured pollen, which is pollinated to Dendrobium nobile in Mount Emei by conventional pollination methods.

[0033] A total of 2103 Dendrobium nobile flowers were artificially cross-pollinated. After pollination, the plants grew well, and no adverse symptoms such as leaf wilting occurred. The fruit setting rate was count...

Embodiment 3

[0038] The experiment site is in the imitation wild planting base of Wanglong Town, Chishui City, Guizhou Province. Three-year-old Dendrobium nobile with strong growth and healthy plants is selected. Among them, Dendrobium nobile of Chishui is the female parent, and Dendrobium nobile of Yunnan is the male parent. After development, only 3 to 5 flower buds remain on each Vitex, and the pollen of Dendrobium nobile is collected according to the conventional method, and the pollen is stored in a refrigerator at 0 to 2°C, and taken out of the refrigerator before pollination , cultivated at 14° C. for 2 h and then at 25° C. for 2 h, the ambient humidity of the cultivation was 80%, and cultured pollen was obtained, and the cultured pollen was pollinated to Dendrobium nobile by conventional pollination methods.

[0039] A total of 820 Dendrobium nobile flowers were artificially cross-pollinated. After pollination, the plants grew well without any adverse symptoms such as leaf wilting. ...

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Abstract

The invention provides an artificial hybridization method for Dendrobium nobile Lindl and belongs to the technical field of Dendrobium nobile Lindl cultivation. The artificial hybridization method includes: culturing the stored pollen of first Dendrobium nobile Lindl at 14-16 DEG C for 1.5-2.5 hours, and culturing at 24-26 DEG C for 1.5-2.5 hours to obtain cultured pollen, and using the cultured pollen to pollinate second Dendrobium nobile Lindl to achieve artificial hybridization of the Dendrobium nobile Lindl, wherein the first Dendrobium nobile Lindl and the second Dendrobium nobile Lindl belong to different Dendrobium nobile Lindl varieties. The method has the advantages that the method increases the fruit setting rate of the Dendrobium nobile Lindl, the fruit setting rate, in the 10thday after natural pollination, of Dendrobium nobile Lindl is 55.4-69.4, the fruit setting rate, in the 10th day after the method is used, of Dendrobium nobile Lindl is 92.3-97.4%, the fruit setting rate, in the 45th day after natural pollination, of Dendrobium nobile Lindl is 39.2-45.6%, the fruit setting rate, in the 45th day after the method is used, of Dendrobium nobile Lindl is 65.8-71.1%, and the fruit setting rate is increased by 20.2-30.9%.

Description

technical field [0001] The invention belongs to the technical field of cultivation of Dendrobium nobile, in particular to a method for artificial hybridization of Dendrobium nobile. Background technique [0002] Dendrobium nobile is a plant of the genus Dendrobium in the Orchidaceae. On the one hand, it has extremely high ornamental value, and it is listed as the most ornamental orchid with Phalaenopsis and Vanda Orchid; on the other hand, it has extremely high medicinal value. It is listed in "Materia Medica It is recorded in "Compendium" that Dendrobium is "the top grade in this classic, sweet, light, and slightly salty. It is mainly used for treating injuries, eliminating numbness and lowering qi, nourishing the five internal organs, strengthening yin and benefiting essence." Based on previous research, Dendrobium nobile It has seven functions of nourishing yin and strengthening yang, alleviating aging, preventing lung and stomach yin deficiency, improving immunity, anti-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 龚记熠乙引洪鲲张宇斌李欲轲
Owner GUIZHOU NORMAL UNIVERSITY
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