Separation and purification method for preparing high-purity spinosad

Abstract

The invention provides a separation and purification method for preparing high-purity spinosad, and relates to the technical field of antibiotic extraction. The method steps include: pretreatment of Saccharopolyspora spinosa fermentation broth, leaching, macroporous resin adsorption, nanofiltration concentration and crystallization. The separation and purification process is optimized, and a pretreatment agent, a leaching solvent, an eluent and a crystallization agent are used, and the amount is optimized; precipitated centrifugal precipitate is subjected to secondary leaching to improve the purity and yield of the spinosad, wherein the purity 97.6% to 98.8%, and the recovery rate is 91.3% to 95.8%. In addition, the operation process of macroporous resin adsorption is optimized, the amountof organic solvent is effectively reduced, the production cost is reduced, the production cycle is shortened, and the process flow is simpler and easier to control.

Description

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AI Technical Summary

Benefits of technology

This new technology helps create better ways to separate and purify Spironene that can be used to treat diseases caused by bacteria or viruses. It also includes methods like extractive solvent treatment before filtration, use of specific chemical substances during evaporation steps, and optimization of these processes based upon factors such as pH value and temperature levels. Overall, this innovative approach simplifies manufacturing while reducing costs compared to previous techniques.

Problems solved by technology

This patented technical problem addressed in this patent relates to improving the efficient isolation and puritation of spinoxadin compounds that have potential pesticides or veterinary uses like agricultural applications. Current techniques involve either expensive processes involving multiple steps or complex operations requiring extensive cleanup procedures. There remains a demand for improved separation and Purification technology that overcomes these issues while maintaining good safety standards associated with their commercial sale.