Microfluidic chip for cell separation and application thereof in tumor cell separation, and cell separation and identification method

Abstract

The present invention relates to a microfluidic chip for cell separation and an application thereof in the tumor cell separation, and a cell separation and identification method. The microfluidic chipcomprises an fluid inlet flow channel, a capture zone and a fluid outlet flow channel, the fluid inlet flow channel is provided with a fluid inlet, the fluid inlet flow channel correspondingly communicates with a fluid inlet side of the capture zone, the fluid outlet flow channel is provided with a fluid outlet, and the fluid outlet flow channel correspondingly communicates with a fluid outlet side of the capture zone; and the capture zone is provided a plurality of capture units and each capture unit is provided with a plurality of flow dividing columns. The microfluidic chip uses a column sorting method, the flow dividing columns play functions of flow dividing and blocking in the flow channels of the capture zone, the blocking of the flow dividing columns respectively forms capture flow channels and passing-through flow channels, target cells to be captured can be trapped in the capture flow channels, while other non-target cells can flow out through the passing-through flow channels, and the whole microfluidic chip is exquisitely designed in structure, can be used for separating the target cells form a cell mixture of a plurality of cells with different cell diameters, is highin separation effect and simple in operation, and can be widely promoted and applied.

Description

technical field [0001] The invention relates to the field of cell detection, in particular to a microfluidic chip for cell separation, its application in tumor cell separation, and a method for cell separation and identification. Background technique [0002] Samples such as peripheral blood contain a variety of cells. How to separate target cells from a variety of cells has always been a research hotspot in the field of cell detection. For example, how to effectively separate and accurately count circulating clonal plasma cells in the peripheral blood of patients with multiple blood cancers, myeloma, is of great significance for the early clinical diagnosis, prognosis and treatment of myeloma . Traditional methods for cell separation include density gradient centrifugation, physical adsorption, cell electrophoresis, immunomagnetic bead method, and flow cytometry. A simple and effective isolation technique for circulating clonal plasma cells in the peripheral blood of pati...

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Problems solved by technology

[0003] Based on this, it is necessary to provide a microfluidic chip for cell separati

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