Anti-human NGF antibody, preparation method and use thereof

Abstract

The present invention provides an antibody or an antigen-binding fragment thereof that binds to NGF, and lists amino acid sequences of the heavy and light chain variable regions of the antibody. The NGF antibody or the antigen-binding fragment thereof provided by the invention has high affinity for NGF, and can effectively block the binding between the NGF receptor and NGF. The antibody or the antigen-binding fragment thereof inhibits the binding activity of NGF and the receptor in vitro, and is suitable for treating pain diseases associated with expression hyperfunction and elevated levels ofNGF.

Description

technical field [0001] The present invention relates to the field of therapeutic monoclonal antibodies, more specifically, to an anti-NGF antibody or an antigen-binding fragment thereof and uses thereof. Background technique [0002] Nerve growth factor (Never Growth Factor, NGF) is the first discovered neurotrophic factor, it has dual biological functions of neuron nutrition and neurite growth, and it has a great influence on the development, differentiation, growth, regeneration and function of central and peripheral neurons. The expression of traits all play an important regulatory role. NGF contains three subunits of α, β and γ, and the β subunit is the active subunit of NGF to play the role of nerve regeneration and repair. Currently known NGF receptors include high-affinity tyrosine kinase receptor (Tropomyosin receptor kinase A, TrkA) and low-affinity p75 neurotrophin receptor (p75Neurotrophin Receptor, p75NTR). [0003] Although the primary role of NGF was to promo...

AI Technical Summary

Problems solved by technology

The immunogenicity of different CDRs is different, resulting in different antibody tolerance speed and toxicity, which directly affects the efficacy of the drug

On the other hand, the subject's immune response to the antibody itself will form immune complexes, which will alter the pharmacokinetics and produce allergic reactions, etc., eliminating its therapeutic use

Images