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Method for obtaining Chinese population individual age

An individual and crowd technology, applied in the field of obtaining the age of individuals in the Chinese population, can solve problems such as the error of predicted age, and achieve the effect of narrowing the scope of the search, improving the efficiency of solving cases, and increasing the complexity.

Active Publication Date: 2019-06-28
DONGHUA UNIV +1
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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for obtaining the individual age of the Chinese population, which overcomes the defect of relatively large error in predicting age due to improper selection of biomarkers and methods

Method used

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  • Method for obtaining Chinese population individual age
  • Method for obtaining Chinese population individual age
  • Method for obtaining Chinese population individual age

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Experimental program
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Embodiment 1

[0034] 1. Collection of samples and extraction of DNA

[0035]Blood samples were collected from 90 individuals (44 females, 46 males) aged between 1 and 79 years. Collected blood samples were placed in collection tubes containing EDTA and frozen at -20 °C until DNA extraction. Genomic DNA was extracted from 200 μl of peripheral blood using the DNeasy Blood and Tissue Kit (QIAGEN) according to the manufacturer's protocol in a final volume of 100 μl. Finally, the integrity was separated by 1% agarose gel electrophoresis.

[0036] 2. Bisulfite modification

[0037] Using the EZ DNA Methylation-Gold Kit (NO.D5005) kit, a volume of 20 μl of genomic DNA (200 ng) was transformed, wherein unmethylated cytosine was converted to uracil, and methylated cytosine was not changed. Complete conversion of GC-rich DNA within 3 hours. Two heat denaturation steps simplify the conversion of unmethylated cytosines to uracil. The total reaction volume was 10 μl. DNA can be analyzed immediatel...

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Abstract

The invention relates to a method for obtaining a Chinese population individual age. The method comprises the steps that DNA of a sample individual is extracted, hydrosulphite modification of DNA methylation is carried out; the methylation degree of an hTERT gene of the DNA is obtained, a regression model of the methylation degree and the age is established, and the Chinese population individual age can be obtained. The method for obtaining the Chinese population individual age achieves the inference of the individual age by using the blood or blood stain through a reliable method.

Description

technical field [0001] The invention belongs to the field of methods for obtaining age, and in particular relates to a method for obtaining the age of individuals in Chinese population. Background technique [0002] The estimation of individual age has always been one of the important research topics in forensic science. Currently, the assessment of individual age is mainly based on the temporal changes of the physical and chemical properties of tissues such as bones and teeth and various substances in the tissues with age. However, the obtained age inference results are subject to the subjective factors of the evaluators, and the analysis requires intact bones or teeth. Without morphological features left at the site, it is impossible to estimate the age of a biological sample. However, in many cases, only blood or bloodstains are left on the test materials at the scene of the case, and the age of the individual cannot be inferred using the above-mentioned method. [0003...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
Inventor 黄志伟辛叶沈裕虎朱倩史萍王蕾王寒冬陈冉冉张晓宇孔颖颖
Owner DONGHUA UNIV
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