Application of extract from sophora alopecuroides in preparation of medicines for relaxing airway smooth muscle

A technology of airway smooth muscle and Sophora japonica is applied in the field of medicine to achieve the effects of relieving airway hyperresponsiveness, great relaxation effect, and relieving symptoms of airway obstruction

Inactive Publication Date: 2019-07-19
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of extract from sophora alopecuroides in preparation of medicines for relaxing airway smooth muscle
  • Application of extract from sophora alopecuroides in preparation of medicines for relaxing airway smooth muscle
  • Application of extract from sophora alopecuroides in preparation of medicines for relaxing airway smooth muscle

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation of embodiment 1 Sophora Sophorae extract

[0017] Select the dried whole herb of Sophora sophorae as raw material, grind it into powder, add it into ethanol with a volume concentration of 75%, soak it, heat and reflux for extraction 2-4 times, and collect the extract; centrifuge the extract, collect the supernatant, and the supernatant The liquid was evaporated to dryness, and the residue was collected; the residue was dissolved in a hydrochloric acid solution with a mass concentration of 2%, extracted 2-4 times with petroleum ether, the petroleum ether part was separated, and the water part of the lower floor was collected; the pH value of the water part Adjust to 9-11, and then extract with ethyl acetate, collect the extract and evaporate to dryness to obtain the extract, that is, the ethyl acetate extract of Sophora sophora.

Embodiment 2

[0018] Example 2 Sophora Sophorae extract relaxes the pre-contraction of airway smooth muscle induced by high potassium

[0019] 1. In vitro isolation of mouse trachea:

[0020] Male adult BALB / c mice were killed by cervical dislocation, the mouth and limbs were nailed to the dissecting board, the chest cavity was cut open after spraying 75% alcohol, and the heart and lung tissues were exposed. For a whole section of tissue, immediately soak the removed tissue in PSS solution (NaCl 135mM, KCl 5mM, MgCl 2 1mM, CaCl 2 2mM, HEPES 10mM, glucose 10mM, pH 7.4), and then transferred to gel plate for further trimming. Under the SZM stereomicroscope, use Venus scissors to carefully remove the connective tissue around the trachea and the residual blood in the lumen.

[0021] 2. The tension detection method of mouse airway smooth muscle:

[0022] Cut the trachea of ​​the mouse into a tracheal ring about 5mm in length and connect it to the hook. Carefully connect the tension transdu...

Embodiment 3

[0024] Example 3 Sophora Sophorae extract relaxes the pre-contraction of airway smooth muscle induced by acetylcholine

[0025] The remaining steps were the same as in Example 2, except that 80 mM high potassium solution (H-K) was replaced with 100 μM acetylcholine (ACh) solution for pre-stimulation after equilibration.

[0026] like figure 2 , the isolated mouse tracheal ring in 100μM ACh solution has a stable and continuous pre-contraction, and the contraction of the contracted tracheal ring decreases gradually after adding the extract of Sophora japonica ethyl acetate. Increased tracheal ring tension gradually returned to the pre-contraction level. The control group did not induce relaxation of precontracted tracheal rings. It shows that the extract of ethyl acetate of Sophora sophora can completely relax acetylcholine and cause the contraction of airway smooth muscle, and the drug effect is dose-dependent.

[0027] To sum up, it can be seen that the extract of Sophora ...

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Abstract

The invention discloses application of an extract from sophora alopecuroides in preparation of medicines for relaxing the airway smooth muscle. Detection on the muscle tension of tracheal rings isolated from a laboratory mouse discovers that the extract from sophora alopecuroides is a medicinal material with an efficient effect for relaxing the airway smooth muscle, which indicates that sophora alopecuroides is a source for new efficient medicines for preventing and treating asthma. The extract from sophora alopecuroides can be used for relieving the symptom of airway obstruction when an asthma patient is attacked, and has a great popularization value.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of Sophora sophora extract in the preparation of medicine for relaxing airway smooth muscle. Background technique [0002] Asthma is a common multiple chronic respiratory disease, and its pathogenesis is not fully understood yet. At present, there are at least 300 million people in the world, and there are nearly 30 million asthma patients in my country. Asthma has become the second leading cause of death and disability in the world after cancer. According to the latest survey released at the press conference of "my country's Asthma Disease Incidence, Treatment Progress and Asthma Management Ten-Year Progress News Bulletin", from 1990 to 2000, the incidence of asthma in mainland China increased from 0.91% to 1.97%. (more than doubled). The survey data of the three cities of Beijing, Chongqing, and Guangzhou in 2010 showed that the prevalence rates of...

Claims

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Application Information

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IPC IPC(8): A61K36/489A61P11/06
CPCA61K36/489A61K2236/333A61K2236/51A61K2236/53A61P11/06
Inventor 赵平刘佳杨新洲
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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