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Early diagnosis marker for hypertensive intracerebral hemorrhage

A high blood pressure and product technology, applied in disease diagnosis, biological testing, biomaterial analysis, etc., can solve the problems of complicated hematoma, difficult diagnosis, time-consuming and other problems, and achieve the goal of reducing mortality, timely genetic diagnosis, and more accurate genetic diagnosis Effect

Pending Publication Date: 2019-07-30
中国人民解放军联勤保障部队第九八0医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the MRI examination takes a long time, and the patient's vital signs and airway must be monitored during the examination of severe acute cases to prevent accidents.
In addition, the MRI manifestations of hematoma in different periods are also more complicated, which sometimes brings difficulties to the diagnosis

Method used

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  • Early diagnosis marker for hypertensive intracerebral hemorrhage
  • Early diagnosis marker for hypertensive intracerebral hemorrhage
  • Early diagnosis marker for hypertensive intracerebral hemorrhage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening of differentially expressed genes

[0037] 1. Research object

[0038] The clinical information of the study subjects is shown in Table 1.

[0039]

[0040] 2. Extraction of total RNA in blood

[0041] (1) Homogenization

[0042] Take fresh blood (peripheral blood) directly, add 3 times the volume of erythrocyte lysate, mix well, place at room temperature for 10 minutes, and centrifuge at 10,000 rpm for 1 minute. Discard the supernatant thoroughly and collect the white blood cell pellet. Add 1 ml TRIzol per 100-200 μl blood collected leukocyte pellet.

[0043] (2) Phase Separation

[0044] a. After adding TRIzol to the sample, place it at room temperature for 5 minutes to fully lyse the sample.

[0045] b. Add 200 μl of chloroform to every 1ml of TRIzol, vibrate vigorously and mix well, then place at room temperature for 3-5min to allow natural phase separation.

[0046] (3) RNA Precipitation

[0047] a. Centrifuge at 12,000rpm at 4°C for ...

Embodiment 2

[0097] Example 2 Large sample verification of differentially expressed genes

[0098] 1. Research object:

[0099] The screening criteria were the same as in Example 1, 30 patients with hypertensive cerebral hemorrhage and 30 normal subjects.

[0100] 2. Extraction of total RNA in blood

[0101] Step is with embodiment 1.

[0102] 3. Reverse transcription

[0103] 1 μg of total RNA was reverse-transcribed to synthesize cDNA using reverse transcription buffer. Use 25μl reaction system, take 1μg total RNA for each sample as template RNA, add the following components to the PCR tube respectively: DEPC water, 5× reverse transcription buffer, 10mmol / L dNTP, 0.1mmol / l DTT, 30μmmol / l Oligo dT, 200 U / μl M-MLV, template RNA. Incubate at 42°C for 1h, then centrifuge briefly at 72°C for 10min.

[0104] 4. QPCR

[0105] (1) Primer design

[0106] QPCR amplification primers were designed according to the coding sequences of SETD6 gene and GAPDH gene in Genbank, and synthesized by S...

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PUM

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Abstract

The invention discloses application of a SETD6 gene for diagnosing hypertensive intracerebral hemorrhage. The SETD6 gene transcription level in blood of a patient suffering from hypertensive intracerebral hemorrhage has remarkable difference with that of a normal person, and according to the difference, the patient suffering from hypertensive intracerebral hemorrhage and the normal person can be distinguished. The invention further discloses a diagnosis product for hypertensive intracerebral hemorrhage. The diagnosis product can achieve diagnosis of hypertensive intracerebral hemorrhage by detecting the SETD6 gene in blood.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis and relates to a molecular marker for diagnosing hypertensive cerebral hemorrhage, in particular to the application of the molecular marker-SETD6 gene in blood in the preparation of products for diagnosing hypertensive cerebral hemorrhage. Background technique [0002] Hypertensive cerebral hemorrhage (HCH) is a spontaneous cerebrovascular disease in the brain parenchyma caused by the rupture of intracerebral arteries, veins or capillaries. Hypertensive intracerebral hemorrhage is a global disease with high morbidity, disability and mortality, and is a common and serious disease that endangers human health. [0003] Hypertension often leads to pathological changes in small arteries at the base of the brain. The prominent manifestations are hyaline or fibrous degeneration and focal hemorrhage, ischemia, and necrosis on the walls of these small arteries, which weakens the blood vessel walls. Inte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883G01N33/6893C12Q2600/158G01N2800/2871G01N2800/321
Inventor 齐洪武
Owner 中国人民解放军联勤保障部队第九八0医院
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