InDel marker remarkably related to soybean plant branching number and application of marker

A technology of branch number and soybean, which is applied in the field of genetic breeding, can solve the problems of long screening time and no effective screening of soybean plant branch number, so as to improve screening efficiency and screening accuracy, optimize plant morphology and structure, Effect of increasing soybean yield

Active Publication Date: 2019-08-13
安徽豆越农业科技有限公司
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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies in the prior art, and provide a kind of InDel mark and application thereof that are significantly related to the number of branches of soybean plants, to solve the problem that there is no effective screening and screening for the number of branches of soybean plants in the prior art. Technical problems such as long technical time

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  • InDel marker remarkably related to soybean plant branching number and application of marker
  • InDel marker remarkably related to soybean plant branching number and application of marker
  • InDel marker remarkably related to soybean plant branching number and application of marker

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[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0027] In the present embodiment, 12 copies of varieties with a large number of plant branches and 12 copies of varieties with a small number of plant branches among 1600 accurate identifications of national soybean germplasm resources (provided by the Institute of Crop Science, Chinese Academy of Agricultural Sciences) were selected, with a total of 24 copies of materials , the detailed list of materials is shown in Attachment 1.

[0028] Numbering ...

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Abstract

The invention discloses an InDel marker remarkably related to the soybean plant branching number and application of the marker, and relates to the technical field of genetic breeding. PCR amplification is conducted on soybean genome DNA with the sequences of SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4 and SEQ ID NO.5 as the primers, amplification products are judged, if the amplification products only include one main band, accordingly there is nothing wrong with to-be-detected soybean DNA and the plant branching number is large, and if the amplification products include two main bands, accordinglythere is nothing wrong with the to-be-detected soybean DNA and the plant branching number is small. By means of the method, the species with the large/small soybean plant branching number can be effectively and rapidly screened out, the first step of screening out the excellent soybean plant material is realized, and the method foundation is provided for deeply carrying out the gene function research and soybean branching number molecular improvement.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to an InDel marker significantly related to the number of branches of soybean plants and a method for identifying the number of branches of soybean plants. Background technique [0002] Soybean is the most important economic oil crop in the world, and it is also the largest source of edible oil and protein. As demand for soybeans grows, it will become increasingly important to use genetic improvement of soybean varieties to increase soybean yield. Optimizing plant morphological structure and cultivating soybean varieties with optimal branching are the breeding goals that plant breeders have been pursuing. [0003] Conventional breeding has a long period of time, low efficiency and high cost, while molecular markers reflect the differences of individual genome DNA fragments, directly reflect the essence of germplasm resources, have the advantages of high efficiency, accurac...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 王晓波万明月张阴王伟邵文韬程安东李佳佳付广辉张玉坤邱丽娟
Owner 安徽豆越农业科技有限公司
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