Method for obtaining seedlings of Actinidia kolomikta
A technology for seedlings of kiwifruit and seedlings, which is applied in the field of seedlings of kiwifruit, and can solve the problems of low germination rate and difficult propagation of kiwifruit seeds
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Embodiment 1
[0042] Embodiment 1, establishment of the method for fast obtaining Actinidia kiwifruit seedlings
[0043] 1. Materials and methods
[0044] 1. Experimental materials
[0045] The Gouzao kiwifruit fruit was picked in Qili, Hengdaohezi on August 13, 2017 and July 26, 2018. The picked fruit was soaked in clean water, the seeds were washed out, the skin and pulp were filtered out, and it was naturally exposed to the sun. Let dry and set aside.
[0046] 2. Experimental method
[0047] (1) Disinfection of kiwi fruit seeds
[0048] Soak kiwifruit seeds in clean water for 48 hours, sterilize with 30% (V / V) hydrogen peroxide for 20 minutes, wash with sterile water twice, soak in 70% (V / V) ethanol for 40 seconds, wash with sterile water for 3 times and set aside.
[0049] (2) Preparation of induction medium for kiwifruit seedlings
[0050] Prepare the following respectively:
[0051] A: Add agar 7g / L, sucrose 20g / L
[0052]B: Add MS (Qingdao Haibo Biotechnology Co., Ltd., produc...
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