Application of glucocorticoid receptor accelerator antcin A in inhibiting tumorigenesis and metastasis of breast cancer

A technology for glucocorticoids and breast cancer tumors. It is applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, and medical raw materials derived from fungi. It can solve the problems of not being able to meet the needs of users, and little understanding of miRNA regulation.

Inactive Publication Date: 2019-09-10
TAIWAN LEADER BIOTECH CORP
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Problems solved by technology

Furthermore, little is known about the regulation of miRNAs through GR promoter-mediated sign...

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  • Application of glucocorticoid receptor accelerator antcin A in inhibiting tumorigenesis and metastasis of breast cancer
  • Application of glucocorticoid receptor accelerator antcin A in inhibiting tumorigenesis and metastasis of breast cancer
  • Application of glucocorticoid receptor accelerator antcin A in inhibiting tumorigenesis and metastasis of breast cancer

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preparation example

[0046] Preparation example: materials and methods

[0047] 1. Chemical agents:

[0048] As previously mentioned, ATA was isolated from the fruiting bodies of Antrodia Cinnamomea by the method described in a previously published study [21]. According to high performance liquid chromatography (HPLC) and mass spectrometer (1H-NMR) analysis, it is confirmed that the purity of ATA is higher than 99%. DMEM medium (Dulbecco's modified Eagle's medium) was purchased from Invitrogen (Carlsbad, CA). RPMI medium (Roswell Park Memorial Institute), minimum essential medium, fetal bovine serum (FBS), sodium pyruvate, penicillin, streptomycin , dexamethasone (DEX), thiazolium blue (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT), 4',6-diamidino-2 -Phenylindole (4',6-diamidino-2-phenylindole, DAPI) and cycloheximide were purchased from Sigma-Aldrich (St. Louis, CA). Antibodies used in the present invention are listed in Table 1. All other chemicals were reagent grade ...

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Abstract

An application of a glucocorticoid receptor accelerator antcin A in inhibiting tumorigenesis and metastasis of breast cancer is disclosed, human breast cancer cells MCF-7 and MDA-MB-231 are treated byutilizing natural glucocorticoid analog antcin A, and then an miR-708 expression is significantly increased by activating a glucocorticoid receptor [alpha] (GR[alpha]). Induction of the miR-708 expression by a GR accelerant can effectively inhibit proliferation of human breast cancer cells, a cell cycle progression, and tumor stem cell expression and cancer cell metastasis. In addition, the GR accelerant or an miR-708 transfection treatment cell significantly inhibits an expression of IKK[belta], also inhibits the activity of NF-[kappa]B and the expression of its downstream target genes, andimproves amount of expression of involvement in cancer cell proliferation, the cell cycle progression, metastasis and cancer stem cells marker proteins p21<CIP1> and p27<KIP1>. Human breast cancer cell xenograft model tests find that treatment of animals with the GR accelerant can significantly inhibit tumor growth and tumor weight and volume. As a whole, a result of an experiment strongly speculates that the GR accelerant induces the miR-708 expression and inhibits NF-[kappa]B downstream signal transduction, and can be a novel treatment mode applied to breast cancer treatment.

Description

technical field [0001] The present invention relates to a glucocorticoid receptor promoter for inhibiting breast cancer tumorigenesis and metastasis, in particular to a synthetic or natural glucocorticoid receptor (GR) promoter that can induce microribose Nucleic acid-708 (microRNA-708, miR-708) expression, thereby inhibiting the occurrence and metastasis of breast cancer tumors, especially activating miR-708 through transcriptional activation of glucocorticoid receptor α (GRα), resulting in decreased proliferation of breast cancer cells and induction of cell cycle Stasis, mainly through the transcriptional repression of NF-κB and its target genes COX-2 and cMYC. Background technique [0002] Breast cancer is the most common malignancy and the leading cause of cancer-related death among women worldwide, with approximately 1.3 million new cases of breast cancer diagnosed each year and an estimated 500,000 deaths from breast cancer [1], according to the International Agency fo...

Claims

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Application Information

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IPC IPC(8): A61K31/575A61P35/00A61P35/04
CPCA61K31/575A61K36/07A61P15/00A61P35/00A61P35/04A61P43/00
Inventor 王升阳林进忠
Owner TAIWAN LEADER BIOTECH CORP
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