Detection reagent and detection method of anthraquinone compound

Abstract

Embodiments of the invention provide a detection reagent and a detection method of an anthraquinone compound. The detection reagent comprises a hydrogen bond acceptor and a hydrogen bond donor corresponding to the hydrogen bond acceptor, wherein the hydrogen bond acceptor comprises L-menthol; and the hydrogen bond donor comprises acetic acid. The detection method comprises the following steps: heating a to-be-detected solution; adding the detection reagent into the to-be-detected solution; performing ice bath after uniform mixing to obtain a solidified layer and a liquid layer; after the liquid layer is poured out, adding a deflocculating agent into the solidified layer for deflocculating; and measuring the content of the anthraquinone compound by HPLC. According to the detection reagent and the detection method provided by the embodiments of the invention, tedious centrifugation and filtration are reduced; on the basis of reserving the ion advantages, the detection reagent and the detection method have the characteristics of low cost, environmental protection, high efficiency and the like; and in addition, the application of DESs also avoids the use of a demulsifier, so that the problem that the mass spectrometry detection cost of the anthraquinone compound is high in the prior art is solved.

Description

technical field [0001] The invention belongs to the technical field of detection of quinone compounds, and in particular relates to a detection reagent and a detection method of anthraquinone compounds. Background technique [0002] Anthraquinones are widely distributed in Polygonaceae, Fabaceae, Rhamnaceae, Rubiaceae and Liliaceae plants, and have various pharmacological effects such as purging, antibacterial and anti-inflammatory, anticancer and antitumor. However, due to the fact that anthraquinones such as emodin, rhein and emodin methyl ether can inhibit the proliferation of HK-2 cells, the leakage rate of lactate dehydrogenase increases, the cells become vacuolated, the mitochondrial membrane potential decreases, and the cells appear obvious Long-term use of anthraquinones will produce reversible liver and kidney toxicity, especially kidney toxicity. Long-term use of anthraquinones can also damage the intestinal mucosal barrier, promote the release of pro-inflammatory...

AI Technical Summary

Problems solved by technology

[0005] One of the embodiments of the present invention provides a detection reagent for anthraquinone compounds, the detection reagent includes a hydrogen bond acceptor and a hydrogen bond donor corresponding to the hydrogen bond acceptor, and the hydrogen bond acceptor includes L - Menthol, the hydrogen bond donor includes acetic acid, which solves the problem of high cost of mass spectrometry detection of anthraquinone substances in the prior art

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