Bacillus CCPM7650 with strong anticancer activity and application thereof

A technology of bacillus and anticancer activity, applied in the field of biopharmaceuticals, can solve the problems of large side effects of anticancer drugs and achieve broad-spectrum anticancer activity

Active Publication Date: 2019-09-24
深圳市人田谷生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the problem of large side effects of existing anticancer drugs, the...

Method used

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  • Bacillus CCPM7650 with strong anticancer activity and application thereof
  • Bacillus CCPM7650 with strong anticancer activity and application thereof
  • Bacillus CCPM7650 with strong anticancer activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A strain of Bacillus CCPM7650 with strong anticancer activity, which belongs to the genus Bacillus and represents a previously unknown bacterial species, has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No.17113, the date of deposit is January 4, 2019, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

Embodiment 2

[0032] This example provides a screening method for Bacillus CCPM7650.

[0033] 1. Preparation method of screening medium and related solutions:

[0034] (1) GRC001 solid medium formula (1L):

[0035] Starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O0.01g, agar 20g; dissolve or suspend starch first, then add other ingredients, shake well after high pressure. The starch in this example is from potato or maize or soybean or millet or other grain or other plant.

[0036] (2) Potassium dichromate: The final concentration of the solution is 50mg / L. The concentration of the storage solution is: 5g / L, the solvent is water, and it is prepared immediately after use, protected from light, and sterilized by filtration. Toxic, do not touch the skin or inhale; when preparing 100mL GRC001 solid medium, add 1mL potassium dichromate stock solution, add it when the medium is not too hot, and mix well.

[0037] (3) FeSO 4 ·7H 2 O mother liquor: ...

Embodiment 3

[0046] The present embodiment provides a kind of amplification method of bacillus CCPM7650:

[0047] Take a single colony from the plate into 5mL GRC001 liquid medium, 120 rpm, 28°C, amplify for 2 to 10 days, then transfer 5mL bacterial liquid to 100mL GRC001 liquid medium, 120 rpm, 28°C , a large number of amplification for 2 to 10 days, and finally the bacterial liquid was centrifuged at 8000rpm / min for 10min, and the supernatant was taken for subsequent anticancer experiments.

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Abstract

The invention relates to bacillus CCPM7650 with strong anticancer activity and application thereof, and belongs to the technical field of biopharmaceuticals. In order to solve the problem of large side effects of existing anticancer drugs, the bacillus CCPM7650 with strong anticancer activity is provided, belongs to bacillus, and is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No.17113; the strain is derived from the intestinal tract of the human body, is a kind of newly-discovered bacillus, and can produce anticancer active metabolites, and the metabolites of the strain can be utilized for treating cancers. Since the strain is discovered in the intestinal tract of the human body, the strain has low or no side effects on the human body, solves the problems of large side effects, poor compliance to patients and the like of the existing anticancer drugs, and is expected to achieve the purpose of preventing the cancers by adjusting the balance of intestinal florae.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a bacillus CCPM7650 with strong anticancer activity and its application. Background technique [0002] With the aging of the population and the prevalence of unhealthy lifestyles, the global cancer burden is becoming more and more serious, and cancer has become the main cause of death. It has the characteristics of high morbidity and high mortality, and is affected by many factors such as genes, environment, and emotions. Impact. At present, chemotherapy and radiotherapy are the main treatments for cancer, supplemented by immunotherapy. Among them, chemotherapy and radiotherapy are the most common methods for treating cancer. However, long-term chemotherapy and radiotherapy will cause patients to develop tolerance, and then cause cancer recurrence, resulting in a significant increase in the five-year survival rate of patients. reduce. In addition, the new...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P35/00A61P35/02C12R1/07
CPCA61K35/742A61P35/00A61P35/02C12N1/20C12N1/205C12R2001/07
Inventor 刘树林
Owner 深圳市人田谷生物医药科技有限公司
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