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Aqueous extracts of Chinese herbs and composition and application thereof

A water-based, biological technology, applied in the direction of microorganisms, animal cells, tissue culture, etc., can solve the problem that patients cannot be treated smoothly

Pending Publication Date: 2019-10-11
MARIA VON MED BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above cases, the patient may not be able to carry out the treatment smoothly

Method used

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  • Aqueous extracts of Chinese herbs and composition and application thereof
  • Aqueous extracts of Chinese herbs and composition and application thereof
  • Aqueous extracts of Chinese herbs and composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0062] Preparation Example 1: Primary culture of human dermal fibroblasts

[0063] Skin samples were obtained with the subject's consent. Skin samples were cut into skin fragments 0.5 to 1 mm cubic in size. Subsequently, the skin fragments were placed in DMEM containing FBS, collagenase and dispase, and incubated at 37° C. for 16 to 18 hours. Centrifugation was performed at 1,500 rpm for 5 minutes. Then, the supernatant was removed to replace the broken cells and skin pieces supplemented with FBS, and the broken skin pieces and cells were evenly distributed in Erlenmeyer flasks, and cultured at 37°C. Cells were subcultured when the cell growth density reached 80%. The cells of the third to sixth passages were used for the experiments described below.

preparation example 2

[0064] Preparation Example 2: Preparation of Chinese herbal medicine extract

[0065] Astragalus and Alisma were purchased from traditional Chinese medicine shops. Earth dragons were purchased from area fishing supply stores. Bitter melon was purchased from the regional fruit and vegetable market.

[0066] 100 grams of Astragalus polyphylla was added to 500 grams of secondary deionized water, followed by boiling at normal pressure for 2 hours. Weigh to get 210 grams of Astragalus polysequence suspension.

[0067] 100 grams of Alisma was added to 500 grams of secondary deionized water, followed by boiling under normal pressure for 2 hours. Weigh 242 grams of Alisma suspension.

[0068] 100 grams of balsam pear fruit was added to 500 grams of secondary deionized water, followed by boiling at normal pressure for 2 hours. Weigh 150 grams of bitter gourd suspension.

[0069] 100 grams of unprocessed whole worms of Earthworm were added to 500 grams of secondary deionized water...

Embodiment 1

[0072] Example 1: Unilateral treatment of cells with extracts

[0073] Take quantitative fibroblasts obtained in Preparation Example 1, plant them in a 96-well flat culture dish, and in the presence of the individual Chinese herbal medicine extracts prepared in Preparation Example 2, culture them in a medium containing 0.5% heat-deactivated calf serum (FBS; Gibco, NY, USA) in Duchenne's Modified Eagle's Medium (DMEM; Sigma Chemical Co., St. Louis, MO, USA) for 3 to 6 days. The control group was the fibroblasts of Preparation Example 1 cultured in DMEM containing 0.5% or 1.0% FBS. The supernatant and cells were harvested and analyzed separately.

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Abstract

The invention discloses aqueous extracts of Chinese herbs and a composition and application thereof, and particularly relates to aqueous extracts of Hedysarum polybotrys Hand.-Mazz., Alisma plantago-aquatica (Sam.) Juzep, Momordica charantia L. or Eisenia foetida, a composition of the extracts and new application in cell culture. The aqueous extracts of the Chinese herbs can replace parts of the functions of animal serum in a cell culture medium, correspondingly the dosage of the animal serum in the cell culture medium can be reduced, the production cost of cells is reduced, and the health risk caused by the use of the animal serum is reduced. The invention also relates to an anti-wrinkle and anti-skin aging cosmetic additive containing the aqueous extracts of the Chinese herbs.

Description

technical field [0001] The present invention relates to new uses of aqueous extracts of Chinese herbal medicines, especially aqueous extracts of a single Chinese herbal medicine, or a combination of aqueous extracts of several Chinese herbal medicines, new compositions and new uses in cell culture, and in New compositions and uses in the fight against skin wrinkles and skin aging. Background technique [0002] Fibroblasts exist in many tissues in animals, and are used to synthesize extracellular matrix and collagen, maintain the basic structure of connective tissue, and play an important role in wound healing. Fibroblasts also respond to migration and proliferation due to the cytokines secreted by themselves or surrounding cells. [0003] The cultured fibroblasts have the effect of promoting tissue repair and can be used in a wide range of applications, including acute and chronic wounds, as well as cosmetic and reconstructive surgery, all of which have good clinical result...

Claims

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Application Information

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IPC IPC(8): C12N5/071A61K8/98A61K8/9789A61Q19/08
CPCC12N5/0602A61K8/987A61K8/9789A61Q19/08C12N2500/76C12N2500/82
Inventor 黄美月
Owner MARIA VON MED BIOTECH
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