Kras gene mutation detection method based on solid-state nanopore colon cancer

Abstract

The invention discloses a kras gene mutation detection method based on solid-state nanopore colon cancer, wherein the method includes the following steps: A, cleaning a fluid device and a gasket, andblowing to dry for standby application; B, assembling nanopores at the front and the back with the gasket and the fluid device respectively; C, forming small chambers by the nanopore chips after completion of assembly; D, injecting a 1 M / 1M KCl solution into the small chambers of the nanopore chips; E, measuring the current and voltage of the nanopores before and after modification; and F, addinga K-ras oncogene fragment of a target base into the solution of a cis surface. A mutant gene codon is subjected to point mutation, resulting in the loss of an enzyme cutting site, so the mutant gene codon cannot be cut off; the mutant gene codon is subjected to point mutation, resulting in the loss of an enzyme cutting site, so the mutant gene codon cannot be cut off. By voltage feedback control,the mutant K-ras gene and a wild-type K-ras gene have electrical output signals measured, and the mutation of the K-ras gene is detected by measuring the change of the ionic current.

Description

technical field

[0001] The invention relates to the technical field of detection of kras gene mutation of colon cancer based on solid-state nanopore, in particular to a detection method of kras gene mutation of colon cancer based on solid-state nanopore. Background technique

[0002] Colon cancer is a common malignant tumor of the digestive tract that occurs in the colon. It is more likely to occur at the junction of the rectum and the sigmoid colon. Its incidence rate ranks third among gastrointestinal tumors. Colon cancer is mainly adenocarcinoma, mucinous adenocarcinoma, undifferentiated carcinoma. The general shape is polypoid, ulcerous and so on. Colon cancer can develop circularly along the intestinal wall, spread up and down along the longitudinal diameter of the intestinal canal, or infiltrate deep into the intestinal wall. In addition to transferring through lymphatic vessels, blood flow, and local invasion, colon cancer can also grow into the abdominal cavity or ...

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