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Colon cancer K-ras oncogene mutation detection system

A detection system and technology for colon cancer, which are applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problem of invalidity in patients with K-ras mutation, and achieve the effect of highlighting the difference of sample signals and improving the signal-to-noise ratio.

Pending Publication Date: 2019-11-05
苏州丽纳芯生物科技有限公司
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Problems solved by technology

Clinically targeted new drugs Erbitux (Erbitux) and Panitumumab (Panitumumab) are only suitable for patients with wild-type K-ras genes without mutations, and are ineffective for patients with K-ras mutations

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  • Colon cancer K-ras oncogene mutation detection system
  • Colon cancer K-ras oncogene mutation detection system
  • Colon cancer K-ras oncogene mutation detection system

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Embodiment Construction

[0052] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0053] see Figure 1-8 , the present invention provides a technical solution: a colon cancer K-ras oncogene mutation detection system, characterized in that: comprising the following steps:

[0054] A, preparing nanopores;

[0055] B. Nanopore characterization and sequence design;

[0056] C. Construction of colon cancer K-ras oncogene detection;

[0057] D. Extraction of nanopore background noise signal;

[0058] E. Feasibility analysis of the application...

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Abstract

The invention discloses a colon cancer K-ras oncogene mutation detection system, wherein the system includes the following steps: A, preparation of nanopores; B, nanopore characterization and sequencedesign; C, construction of colon cancer K-ras oncogene detection; D, extraction of nanopore background noise signals; E, feasibility analysis of a nanopore technology applied to colon cancer K-ras gene mutation detection; and F, establishment of a sample model. The system focuses on the advantages of stable solid-state nanopores, easy design and processing, high throughput and easy physical and chemical modification. An MvaI enzyme digestion site is introduced at a codon 12 of a wild-type K-ras oncogene, and translocation behavior of mutation of the colon cancer K-ras gene after enzyme digestion and the wild type in a nano-scale environment is studied by using a highly sensitive nanopore ion current detection technology, the law of molecular motion is revealed and the dynamic mechanism isexplained.

Description

technical field [0001] The invention relates to the technical field of K-ras oncogene mutation detection, in particular to a colon cancer K-ras oncogene mutation detection system. Background technique [0002] Colon cancer is a common malignant tumor of the digestive tract that occurs in the colon. It is more likely to occur at the junction of the rectum and the sigmoid colon. Its incidence rate ranks third among gastrointestinal tumors. Colon cancer is mainly adenocarcinoma, mucinous adenocarcinoma, undifferentiated carcinoma. The general shape is polypoid, ulcerous and so on. Colon cancer can develop circularly along the intestinal wall, spread up and down along the longitudinal diameter of the intestinal canal, or infiltrate deep into the intestinal wall. In addition to transferring through lymphatic vessels, blood flow, and local invasion, colon cancer can also grow into the abdominal cavity or spread along the suture or incision surface . Patients with chronic colit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/682
CPCC12Q1/6886C12Q1/682C12Q2600/156C12Q2565/631
Inventor 谭生伟
Owner 苏州丽纳芯生物科技有限公司
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