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High-throughput str typing system and kit for identifying complex genetic relationships

A kind of kinship and kit technology, which can be used in the determination/examination of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve problems such as inability to distinguish complex kinship relationships

Active Publication Date: 2020-06-05
SHANXI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most linked STR studies are X-STR and Y-STR genetic markers, which require special kinship to be applied; independently inherited autosomal STRs cannot distinguish complex kinship

Method used

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  • High-throughput str typing system and kit for identifying complex genetic relationships
  • High-throughput str typing system and kit for identifying complex genetic relationships
  • High-throughput str typing system and kit for identifying complex genetic relationships

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: A STR typing system and its kit for complex genetic relationship identification

[0079] 1.1 Composition and characteristics of autosomal-linked STR loci:

[0080] (1) The STR typing system in this embodiment comprises the following 60 STR loci:

[0081] D1S2131、D1S3721、D1S2130、D1S1600、D1S1653、D1S1660、D1S3732、D2S1364、D2S2734、D2S1396、D2S428、D2S435、D2S1387、D2S1792、D2S1399、D2S2959、D3S2431、D3S4547、D4S1643、D4S2408、D4S3326、D4S2368、D5S2845、D5S1473、D5S813、 D5S1716、D5S1459、D5S1487、D5S1466、D5S2496、D5S2501、D6S1019、D6S2417、D6S2412、D6S1284、D7S1820、D7S3050、D7S821、D8S594、D8S1468、D8S2322、D8S569、D8S1475、D9S746、D9S319、D9S2149、D11S2010、D11S1392、D12S376、D12S1052、 D13S317, D13S790, D14S1432, D14S1428, D15S644, D15S1507, D16S752, D16S485, D20S481 and D20S1151;

[0082] It has the following measurable properties:

[0083] 1) The repeating unit of the core region is a tetranucleotide;

[0084] 2) The heterozygosity of each STR is greater than 0.6;

[0085] 3) Each group contain...

Embodiment 2

[0105] Example 2: A STR typing system and its kit for complex genetic relationship identification

[0106] 2.1 Composition and characteristics of autosomal-linked STR loci:

[0107] (1) The STR typing system in this embodiment comprises the following 58 STR loci:

[0108]D1S3736、D1S1665、D1S2127、D1S2138、D1S1642、D1S518、D1S1604、D1S3729、D1S3727、D2S1336、D2S1779、D2S1771、D2S437、D2S2970、D2S2944、D2S1327、D3S2402、D3S1766、D4S2411、D4S3351、D4S243、D4S2426、D4S2373、D5S1490、D5S2856、 D5S2495、D5S2855、D5S1722、D5S1725、D6S1048、D6S1275、D7S796、D7S1799、D8S2324、D8S2330、D8S1144、D9S745、D9S301、D9S1124、D10S1246、D10S2485、D11S1983、D11S2363、D11S1368、D12S393、D12S1063、D13S1807、D13S1492、D14S738、D14S301、 D14S583, D15S816, D15S1514, D18S872, D18S972, D18S548, D20S1145, D20S477;

[0109] It has the following measurable properties:

[0110] 1) The repeating unit of the core region is tetranucleotide;

[0111] 2) The heterozygosity of each STR is greater than 0.6;

[0112] 3) Each group contains at least two STRs...

Embodiment 3

[0125] Example 3: A STR typing system and its kit for complex genetic relationship identification

[0126] 3.1 Composition and characteristics of autosomal-linked STR loci:

[0127] (1) The STR typing system in this embodiment comprises the following 61 STR loci:

[0128] D1S532、D1S1611、D1S3733、D1S533、D1S1614、D2S2977、D2S1374、D2S1394、D2S2966、D2S2969、D2S1371、D2S434、D2S1338、D3S4016、D3S2388、D4S1626、D4S1653、D5S2858、D5S2796、D5S1463、D5S815、D5S2499、D5S2498、D6S1043、D6S1274、 D6S1056、D6S1013、D6S1054、D7S820、D7S2205、D7S3071、D7S2845、D8S2326、D8S1464、D8S2320、D8S1470、D8S588、D8S1471、D9S2026、D9S747、D9S2128、D10S2469、D10S1238、D11S4464、D11S4958、D13S1818、D13S767、D14S615、D14S608、D14S597、 D14S302, D14S749, D16S767, D16S3393, D18S537, D18S875, D18S1367, D20S1152, D20S206, D20S607, D20S1146;

[0129] It has the following measurable properties:

[0130] 1) The repeating unit of the core region is tetranucleotide;

[0131] 2) The heterozygosity of each STR is greater than 0.6;

[0132] 3) Each group ...

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Abstract

The invention discloses a high-throughput STR typing system capable of identifying complex consanguinity and a kit thereof, and relates to the technical field of nucleic acid detection in vitro. The high-throughput STR typing system capable of identifying complex consanguinity comprises PCR primers used for amplifying 60, 118 or 179 linked autosomal STR loci; and the kit comprises a PCR primer composition, an Index connector sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer solution B, a database setting-up reagent and the like. The high-throughput STRtyping system capable of identifying complex consanguinity and the kit thereof provided by the invention are capable of realizing single-tube amplification of 60, 118 or 179 linked autosomal STR loci; and moreover, the STR typing system and the kit thereof are good in balance, high in sensitivity, good in specificity, and accurate in typing result. Thus, the high-throughput STR typing system capable of identifying complex consanguinity and the kit thereof can be used for identifying complex relationships of different consanguinity at the same level.

Description

technical field [0001] The invention relates to the technical field of forensic detection, in particular to a high-throughput STR typing system and a typing kit for complex genetic relationship identification. Background technique [0002] Short tandem repeat (short tandem repeat, STR) is a special sequence composed of 2-6bp repeating units in series. The number of STR loci is large and widely distributed, accounting for about 3% of the entire genome, and its polymorphism is high. It is mainly derived from the difference in the number of core sequence repeats among individuals, which follows the Mendelian law of inheritance in the genetic process. Therefore, STR amplification detection technology is widely used in forensic individual identification, kinship identification and population genetics research. The most commonly used STR typing techniques are fluorescent labeling multiplex amplification combined with capillary electrophoresis (capillary electrophoresis, CE) typin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 严江伟范庆炜张更谦张君程凤王萌春李万婷石林玉张晓梦张家榕
Owner SHANXI MEDICAL UNIV
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