Metabolite liquid chromatogram-mass spectrometry detection method

A liquid chromatography and detection method technology, which is applied in the field of substance detection, can solve problems such as excessive sample content and exceeding the detection threshold of equipment, and achieve the effects of improving experimental efficiency, reducing data analysis and time, and ensuring accuracy and effectiveness

Active Publication Date: 2019-12-20
嘉兴迈维代谢生物科技有限公司
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Problems solved by technology

[0003] In the detection by liquid chromatography-mass spectrometry system, due to the uncertain performance of the content of the substance in the sample, it is often prone to the phenomenon that the sample content is too high to exceed the detection threshold of the equipment

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[0031] In order to make the above objects, features and advantages of the present invention more comprehensible, the specific implementation of the present invention will be described in detail below in conjunction with specific examples.

[0032] In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do it without departing from the meaning of the present invention. By analogy, the present invention is therefore not limited to the specific examples disclosed below.

[0033] Second, "one embodiment" or "an embodiment" referred to herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. "In one embodiment" appearing in different places in this specification does not all refer to the same embodime...

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Abstract

The invention discloses a metabolite liquid chromatogram-mass spectrometry detection method. The method comprises the following steps of firstly detecting a to-be-detected sample on a computer; importing a standard substance detection database file; preparing the first detection result off-line data of the to-be-detected sample; preparing DP (declustering potential) and CE (collision energy) combined gradient information; setting a peak height threshold; exporting DP and CE optimization results; and establishing DP and CE optimized databases according to the optimization detection results. Through the detection method disclosed by the invention, the time consumed for conventional DPCE project optimization condition analysis and groping is greatly reduced; the sample collection condition can be accurately monitored by taking 2-chlorophenylalanine as an internal standard, and a fluctuation state of an instrument is monitored by taking 17 mixed standards as the quality control products. The data analysis and time are greatly reduced, the accurate monitoring on the adoption process and the instrument fluctuation is realized, and an experiment efficiency of the liquid chromatogram-massspectrometry detection is improved.

Description

technical field [0001] The invention belongs to the technical field of substance detection, and in particular relates to a liquid chromatography-mass spectrometry detection method for metabolites. Background technique [0002] Metabolomics is a discipline similar to genomics and proteomics, which conducts large-scale qualitative and quantitative analysis of metabolites in organisms, and uses this to find out the relationship between each metabolite. [0003] In the detection by liquid chromatography-mass spectrometry system, due to the uncertainty of the content of substances in the sample, it is often prone to the phenomenon that the sample content is too high to exceed the detection threshold of the equipment. By adjusting the declustering potential (DP) and collision energy (collision energy, CE) of each target substance, the threshold value of the detection substance was adjusted to the effective detection range. The adjustment of DP and CE not only requires the operato...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045
Inventor 唐堂高猛董学奎王宏郑彬石坚
Owner 嘉兴迈维代谢生物科技有限公司
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