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Use of detection reagent of CHAMP1 autoantibody in preparation of lung cancer screening kit

A technology of autoantibodies and kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of no CHAMP1 protein, no lung cancer, etc., and achieve good application prospects, effective screening, and low patient harm Effect

Inactive Publication Date: 2019-12-31
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no related reports on CHAMP1 protein autoantibodies, and there is no prior art related to lung cancer

Method used

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  • Use of detection reagent of CHAMP1 autoantibody in preparation of lung cancer screening kit
  • Use of detection reagent of CHAMP1 autoantibody in preparation of lung cancer screening kit
  • Use of detection reagent of CHAMP1 autoantibody in preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Relationship Between CHAMP1 Autoantibodies in Plasma and Lung Cancer

[0028] 1. Clinical data

[0029] Select 30 cases of lung cancer patients, 29 cases of benign lung diseases, and 29 cases of healthy controls. The basic information is as follows:

[0030] Basic Information lung cancer patients benign lung disease healthy control number of people 30 29 29 age 49.5±5.7 46.5±10 42.0±8.9 male ratio 20(66.7％) 12(41.4％) 13(46.7％)

[0031] 2. Detection principle

[0032] HuProt TM CHAMP1 protein is immobilized on the human protein custom chip (the CHAMP1 protein used is a full-length protein, and the protein sequence number is ENSP00000474448). After incubation with serum, CHAMP1 autoantibodies (mainly including IgG and IgM antibodies, and some other types Antibodies) will bind to it, wash to remove unbound antibodies and other proteins, and then detect with anti-human IgM fluorescently labeled secondary antibody (cy5...

Embodiment 2

[0049] Embodiment 2 The composition of detection kit of the present invention and its application method

[0050] 1. Kit composition

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Abstract

The invention relates to the field of in vitro diagnostic reagents, and in particular to use of a detection reagent of a CHAMP1 autoantibody in preparation of a lung cancer screening kit. The invention finds that the level of autoantibody of CHAMP1 protein in the serum of lung cancer patients is significantly lower than that in healthy patients for the first time, and can realize effective screening of lung cancer by using the reagent of detecting the autoantibody of CHAMP1 protein for the preparation of a lung cancer screening kit.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of CHAMP1 autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cance...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68G01N33/535
CPCG01N33/535G01N33/57423G01N33/57488G01N33/6854G01N33/6884G01N2333/4746
Inventor 张立李为民
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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