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Application of HECTD3 autoantibody detection reagent in preparing lung cancer screening kit

A technology of autoantibodies and kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of no HECTD3 protein, no lung cancer, etc., and achieve good application prospects, effective screening, and low patient harm Effect

Active Publication Date: 2019-11-05
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no related reports on HECTD3 protein autoantibodies, and there is no prior art related to lung cancer

Method used

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  • Application of HECTD3 autoantibody detection reagent in preparing lung cancer screening kit
  • Application of HECTD3 autoantibody detection reagent in preparing lung cancer screening kit
  • Application of HECTD3 autoantibody detection reagent in preparing lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Relationship between HECTD3 autoantibodies in plasma and lung cancer

[0026] 1. Clinical data

[0027] 30 lung cancer patients and 29 healthy controls were selected. The basic information is as follows:

[0028] Basic Information lung cancer patients healthy control number of people 30 29 age 49.5±5.7 42.0±8.9 male ratio 20(66.7％) 13(44.8％)

[0029] 2. Detection principle

[0030] HuProt TM The HECTD3 protein is immobilized on the human protein custom chip. After incubation with serum, HECTD3 autoantibodies (mainly including IgG, IgM antibodies, and some other types of antibodies) in the serum will bind to it, and the unbound antibodies and other proteins will be removed by washing. Then use anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green) to detect, and read the signal through a fluorescent scanner. The strength of the si...

Embodiment 2

[0047] Embodiment 2 The composition of detection kit of the present invention and its application method

[0048] 1. Kit composition

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Abstract

The invention relates to the field of in-vitro diagnostic reagents, in particular to application of an HECTD3 autoantibody detection reagent in preparing a lung cancer screening kit. According to theapplication, it is found for the first time that the level of HECTD3 protein autoantibodies in serum of a patient with the lung cancer is significantly lower than that of a healthy patient. The reagent for detecting the HECTD3 protein autoantibodies is used for preparing the lung cancer screening kit, and effective screening of the lung cancer can be achieved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of a HECTD3 autoantibody detection reagent in the preparation of a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung can...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6884G01N2800/12
Inventor 王巍
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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