Muscovy duck reovirus cohabitation infection occurrence model and construction method thereof

Abstract

The invention belongs to the technical field of animal infection and discloses a Muscovy duck reovirus cohabitation infection occurrence model and a construction method thereof. The Muscovy duck reovirus cohabitation infection occurrence model is characterized in that susceptible young Muscovy ducks are artificially injected with a 106TCID50 Muscovy duck reovirus YB isolated strain through leg intramuscular injection, injection infected young Muscovy ducks in a certain proportion are taken as infection sources and put into a susceptible experimental young Muscovy duck group to simulate a 'fecal-oral' natural transmission route of Muscovy duck reovirus so as to cause infection of susceptible experimental young Muscovy ducks. According to results, three days later after artificial leg intramuscular injection infection, cohabitation infection is performed under the condition that a ratio of artificial injection infected young Muscovy ducks to cohabitation infected experimental young Muscovy ducks is 2:5, a disease course of the experimental young Muscovy ducks in cohabitation infection with MDRV (Muscovy duck reovirus) has a disease course development process of typical clinical MDRVinfection, and clinical symptoms, anatomy variation, the incidence rate and the fatality rate are uniform with those of clinical natural infection. The practical model is provided for Muscovy duck reovirus infection related research according to the results.

Description

Technical field [0001] The invention belongs to the technical field of animal cells, and particularly relates to a muscovy duck reovirus cohabiting infection pathogenesis model and a construction method thereof. Background technique [0002] At present, the existing technology commonly used in the industry is as follows: Muscovy duck reovirus infection, commonly known as "liver white spot disease" in Muscovy duck reovirus (Muscovy duck reovirus, MDRV) infection, is the immunosuppression of young Muscovy ducks. Sexually transmitted diseases. In the study of the pathogenesis of the disease and the process of prevention and treatment, it is necessary to establish a case of the disease, usually 10 6 TCID 50 MDRV isolates were infected by intramuscular injection of each feather. In the natural state, the infected Muscovy ducks and diseased young Muscovy ducks mainly excrete the virus through the digestive tract, while the susceptible Muscovy ducks are infected with the reovirus throu...

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problems existing in the prior art are: the following deficiencies exist in the experimental animals of the conventional artificial intramuscular injection route for direct infection research: the pathogen directly enters the body without the body surface and mucosal barrier, quickly invades the bloodstream and spreads to the whole body, Arrives at the target tissue or organ in a short time, and the onset is acute. Although the clinical symptoms of the onset are typical, there is no typical incubation period, prodromal period, onset period, and recovery period of natural infection. The regularity of development is not conducive to the study of the pathogenic mechanism and prevention of muscovy duck reovirus infection

Images