Microorganism isolation culture and culture medium supply apparatus

A technology for supplying devices and culture fluids, applied in enzymology/microbiology devices, biochemical cleaning devices, methods for supporting/immobilizing microorganisms, etc., which can solve problems affecting the normal growth of microorganisms, slow dialysis speed, and easy damage to membranes, etc. Achieve the effects of increasing hydraulic pressure difference, reducing filtration volume and improving reliability

Active Publication Date: 2020-01-21
HUAQIAO UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a microbial isolation culture and culture solution supply device, to solve the problems of easy damage of the membrane and slow dialysis speed in the above-mentioned background technology, and to solve the problem of separating the culture solution while affecting the normal growth of microorganisms

Method used

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  • Microorganism isolation culture and culture medium supply apparatus
  • Microorganism isolation culture and culture medium supply apparatus

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Embodiment 1

[0038] Such as figure 1 , a kind of microbial isolation cultivation and culture solution supply device of the present embodiment, the present embodiment takes Acidithiobacillus ferrooxidans (Acidithiobacillus ferrooxidans) as an example to cultivate, and the main nutrient is Fe 2+ , the main metabolite is Fe 3+ , this example utilizes Fe 3+ Oxidation of metals to achieve the removal of metal materials, Fe 3+ Self-reduction to Fe while oxidizing the metal 2+ , so the processed liquid can be recycled. The types of microorganisms 6 applicable to this embodiment are not limited to the cultivation of fungi and algae, but are also applicable to any other suitable types of microorganisms 6 . The term "culture fluid" refers to the liquid in which the target microorganism 6 is cultivated, including water, nutrients required for the cultivation of the microorganism 6, metabolites of the microorganism 6, and the like.

[0039] A microbial isolation culture and culture fluid supply d...

Embodiment 2

[0055] The difference between embodiment 2 and embodiment 1 is that this embodiment also includes a liquid replenishment device 8, which is filled with an appropriate amount of sucrose medium for supplementing the culture liquid consumed in the process of microorganism cultivation.

[0056] Such as figure 2 , the type of microorganism cultured in this embodiment is Saccharomyces cerevisiae (Saccharomycescerevisiae), the main nutrient substance is sucrose, and the main metabolite is alcohol. In this embodiment, the culture solution is separated and (or) purified to finally obtain the alcohol product. Alcohol needs to be supplemented with sucrose medium at the same time. In this embodiment, the supply form is non-recyclable, so the post-processing device 7 does not have a return line to the culture fluid exchanger 2, and the separate cultivation and supply methods are as follows:

[0057] a. Prepare a certain volume of inoculum of yeast in the logarithmic growth phase and a li...

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Abstract

The invention discloses a microorganism isolation culture and culture medium supply apparatus. The apparatus comprises a bioreactor, a culture medium exchanger, and a hydraulic regulating pump. A filter membrane or filter element is selected and used to surround microorganisms in a culture medium space of the culture medium exchanger, and the other part of the culture medium does not contain the microorganisms, so that isolation culture is realized. A pressure difference of positive and negative periodic change is generated on two sides of the filter membrane or filter element by the hydraulicregulating pump, the culture medium is forced to flow through the filter membrane or filter element back and forth, and an exchange speed of nutrients and metabolites in the partitioned space is increased. The culture medium on the non-microorganism side is transported to a designated place by a culture medium transporting pump. Finally the normal growth of the microorganisms is not affected while the culture medium is separated.

Description

technical field [0001] The invention belongs to the field of microorganism cultivation, and in particular relates to a microorganism isolation cultivation and culture solution supply device. Background technique [0002] With the continuous development of industrial technology, especially microbial engineering, the depth and breadth of the application of microorganisms in industrial production has increased to a certain extent. In these applications, it is often not the microbial cell body itself but the metabolites of the microorganism that are used. Therefore, in many cases, it is necessary to separate the culture fluid from the microbial cell population to obtain the culture fluid containing metabolites, which can meet industrial applications. However, ordinary filtration and separation methods are not only time-consuming and laborious, but also affect the normal growth of microorganisms and reduce the yield of metabolites. In order to continuously and stably obtain the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/38C12M1/12
CPCC12M25/00C12M25/02C12M29/04C12M29/20C12M41/22C12M47/10
Inventor 黄辉马飞
Owner HUAQIAO UNIVERSITY
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