Methods for preventing and treating urinary incontinence
A treatment method, a technology for urinary incontinence, applied in chemical instruments and methods, pharmaceutical formulations, urinary system diseases, etc., and can solve problems such as no reported 100% success.
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[0451] general method
[0452] ActRIIB antibodies, their characterization and methods related thereto have been disclosed in WO 2010 / 125003, such as (i) functional assays, (ii) reporter gene assay (RGA), (iii) culture of HEK293T / 17 cell line, (iv) Myostatin-induced luciferase reporter gene assay, (v) specificity ELISA, (vi) ActRIIB / Fc-myostatin binding interaction ELISA, (vii) FACS titration of hActRIIB-expressing and hActRIIA-expressing cells , (viii) binding to primary human skeletal muscle cells, (ix) affinity determination for selected anti-human ActRIIB Fabs using surface plasmon resonance (Biacore), (x) CK assay, (xi) animal model, ( xii) treatment regimen, (xiii) statistical analysis, (xiii) panning, (xv) antibody identification and characterization, (xvi) optimization of antibodies derived from first affinity matured, (xvii) IgG2 conversion of affinity matured Fab (1st maturation), (xviii) second affinity maturation, (xx) IgG2 conversion and characterization of IgG2 (...
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