Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting amount of bacteria

A technology of quantity and bacteria, applied in the field of microbial detection, to improve the accuracy of counting, avoid easy deformation and high recognition rate

Inactive Publication Date: 2020-02-18
湖南航天天麓新材料检测有限责任公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the defects in the direct counting method of microbial microscope in the prior art, the present invention provides a method for detecting the number of bacteria to solve the defects in the direct counting method of microbial microscope in the background technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting amount of bacteria
  • Method for detecting amount of bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The technical solutions in the present invention are clearly and completely described below, obviously, the described embodiments are only some embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0028] Taking commercially available probiotics (2g / branch, 20 billion units of labeled lactic acid bacteria, that is, 10 billion CFU / g) as an example, to illustrate a method for detecting the number of bacteria proposed by the present invention, including the following steps:

[0029] 1. Weigh 0.01g of probiotics, dissolve and dilute the probiotics to a suitable solubility (the solubility is determined by the number of bacteria on the sample stage with conductive glue) with a phosphate buffer solution with a pH value of 7.4, Perform centrifugation.

[0030] The specifi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting amount of bacteria, and relates to the technical field of microorganism detection. The method for detecting amount of bacteria performs a series of processing on the sample before observation and counting, and then uses a scanning electron microscope for image counting. In a series of processing, the fixed processing avoids inaccurate counting causedby bacterial movement during the counting process, and at the same time, can also avoid the problem that the bacterial structure is easily deformed due to air pressure and electron beam bombardment. Using scanning electron microscope for imaging counting can directly use the material properties of the surface material of the sample for microscopic imaging with no requirements for bacterial concentration, and there is basically no limit on the size of the bacteria by being able to be enlarged by dozens to millions of times. At the same time, the scanning electron microscope has a function of measuring the size, which can quickly measure the size of the bacteria, has a high recognition rate, can distinguish microorganisms from tiny debris, and improves counting accuracy by eliminating the tiny debris during counting.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a method for detecting the number of bacteria by imaging with a field emission scanning electron microscope. Background technique [0002] At present, microbial counting methods are divided into direct counting method and indirect counting method. The direct counting method of microbial microscope is to drop the uniform bacterial liquid of microorganisms onto a clean hemocytometer plate, and then place it under a microscope to magnify the microorganisms to an appropriate multiple. Observation, method of counting. [0003] The direct counting method of microbial microscope has the following disadvantages: ①It is not suitable for counting motile bacteria; ②It requires a relatively high concentration of bacteria; ③It is difficult to observe small bacteria under a microscope, and it is only suitable for bacteria or spores with relatively large cells ; ④Th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/2251G01N23/2202
CPCG01N23/2251G01N23/2202
Inventor 贺令娟程菊红伍凤祥
Owner 湖南航天天麓新材料检测有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com