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Preparation method of earthworm specimens

A production method and technology of earthworms, applied in the field of production of earthworm specimens, can solve the problems of specimen damage, insufficient detail and system, and achieve the effect of low cost, easy access, and detailed content

Active Publication Date: 2020-03-13
中科小蚓(广东)科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Standardized specimens can be stored for a long time and have a wide range of uses, and are an important means of authoritative identification of earthworm species. At present, there is no uniform method for the preparation of earthworm specimens. They are all operated according to the simple description in traditional literature. It is not detailed and systematic enough. At the same time, most of them use formalin, phenol and other reagents as antiseptic materials. These reagents will cause certain damage to the specimen itself and the creator. With the continuous advancement and development of science and technology, these aspects need to be improved and improved. Replenish

Method used

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  • Preparation method of earthworm specimens

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Effect test

Embodiment 1

[0025] Embodiment 1: a kind of preparation method of earthworm specimen

[0026] 1. Pre-cultivation: Put the earthworms into the pre-laid wet papyrus container, keep the temperature at 25°C and the humidity at 70%, and put them in the dark to stop eating. After the soil in the intestines of the earthworms is discharged, feed them with crushed wet papyrus or agar Earthworms for 6 days, filling the intestines of earthworms.

[0027] 2. Soaking: Soak the pre-cultured earthworms in an ethanol solution with a mass concentration of 10%, until the back holes of the earthworms will secrete a large amount of mucus.

[0028] 3. Filling and fixation: Take out the soaked earthworms, inject 4% pentylene glycol fixative solution with a mass concentration into the body cavity of the earthworms with a syringe from the rear two ends, and adjust the earthworms to a free-moving state.

[0029] 4. Preservation: Use 2g potassium sorbate, 130mL industrial ethanol, 8mL glycerol, 13g citric acid, 1g...

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Abstract

The invention discloses a preparation method of an earthworm specimen. The preparation method comprises the following steps: pre-culturing earthworms, soaking the earthworms in an ethanol solution, filling and fixing the soaked earthworms with pentanediol, and sealing and storing the earthworms with a storage solution. According to the method, materials are easy to obtain, toxic substances such asmethylbenzene are not contained, environmental protection and safety are achieved, the original physiological structure and appearance of earthworms can be kept, the manufacturing process is simple,and popularization is facilitated.

Description

technical field [0001] The invention belongs to the field of preparation methods of animal specimens, and in particular relates to a preparation method of earthworm specimens. Background technique [0002] Earthworms, also known as earthworms, are one of the most widely distributed and typical soil macrofauna in the soil. They can significantly improve soil microstructure through activities such as feeding, excreting, and digging holes, and affect soil material circulation and energy flow. As "ecosystem engineers", we need to know and understand more. "A specimen is worth a thousand words". As an important means of biological monitoring, protection, classification, scientific research and teaching, specimen production is of great significance for improving the understanding of the relationship between human beings and living things, understanding their distribution and habits, and promoting the development of biology. [0003] Standardized specimens can be stored for a long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/36A01N1/00
CPCG09B23/36A01N1/00
Inventor 刘通李祥东程炯蒋新宇刘平蒙星宇孙传谆
Owner 中科小蚓(广东)科技有限公司
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