A method for removing hexavalent chromium in solution by marine Aspergillus niger

A marine Aspergillus niger, hexavalent chromium technology, applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of non-recyclable use, high cost, small adsorption capacity of Aspergillus niger spores, etc. To achieve the effect of controllable size, convenient preparation and good tolerance

Active Publication Date: 2018-01-16
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese invention patent (CN 104944596 A) discloses a method for treating hexavalent chromium heavy metal wastewater with Aspergillus niger spores. The spores adsorb hexavalent chromium to reduce the hexavalent chromium content in wastewater, but the adsorption amount of Aspergillus niger spores is small, and they cannot be recycled, and the cost of this method is relatively high

Method used

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  • A method for removing hexavalent chromium in solution by marine Aspergillus niger
  • A method for removing hexavalent chromium in solution by marine Aspergillus niger
  • A method for removing hexavalent chromium in solution by marine Aspergillus niger

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Embodiment 1

[0029] Embodiment 1: the cultivation of Aspergillus niger mycelium ball

[0030] Inoculate marine Aspergillus niger on PDA medium (potato 200g / L, glucose 20g / L, agar 17g / L), place it in a constant temperature incubator (25°C) and cultivate it for 3 days, take a certain amount of spores and inoculate it into the preparation culture base (glucose 10g / L, NH 4 Cl2g / L, KH 2 PO 4 2g / L, MgSO 4 0.5g / L, yeast extract powder 2g / L), in constant temperature 25 ℃ and the shaker of rotating speed 100 revs / min, constant temperature culture 60 hours, form the substantially uniform aspergillus niger mycelial ball of size. Collect aspergillus niger mycelia ball, its overall appearance is as follows figure 1 Shown are untreated wet mycelium balls. The mycelial balls of Aspergillus niger were of uniform size and good spherical shape, and the shape remained unchanged after freeze-drying. The specific particle size of Aspergillus niger mycelium balls can be obtained by statistics, and its a...

Embodiment 2

[0032] Embodiment 2: the cultivation of aspergillus niger mycelium ball

[0033] Inoculate marine Aspergillus niger on PDA medium (potato 180g / L, glucose 18g / L, agar 15g / L), place it in a constant temperature incubator (25°C) and cultivate it for 4 days, take a certain amount of spores and inoculate it into the preparation culture base (glucose 8g / L, NH 4 Cl1.8g / L, KH 2 PO 4 1.8g / L, MgSO 4 0.4g / L, yeast extract powder 1.8g / L), in constant temperature 25 ℃ and the shaker of rotating speed 50 revs / min, constant temperature culture 48 hours, form the substantially uniform Aspergillus niger mycelial ball of size. The aspergillus niger mycelia balls were taken out, washed twice with water, aged in clear water for 10 hours to form a biological water treatment agent that can be used to remove hexavalent chromium.

[0034]The prepared biological water treatment agent is directly added to the wastewater containing hexavalent chromium, and is fully mixed by mechanical stirring for...

Embodiment 3

[0035] Embodiment 3: the cultivation of Aspergillus niger mycelium ball

[0036] Inoculate marine Aspergillus niger on PDA medium (potato 220g / L, glucose 22g / L, agar 20g / L), place it in a constant temperature incubator (30°C) and cultivate it for 5 days, take a certain amount of spores and inoculate it into the preparation culture base (glucose 12g / L, NH 4 Cl2.2g / L, KH 2 PO 4 2.2g / L, MgSO 4 0.6g / L, yeast extract powder 2.2g / L), in constant temperature 30 ℃ and the shaker of rotating speed 200 revs / min, constant temperature culture 72 hours, form the substantially uniform Aspergillus niger mycelial ball of size. The Aspergillus niger mycelia balls were taken out, washed twice with water, placed in clear water and aged for 24 hours to form a biological water treatment agent that can be used to remove hexavalent chromium.

[0037] Add the prepared biological water treatment agent directly into the wastewater containing hexavalent chromium, and use a fluid pump to circulate ...

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Abstract

The invention relates to a method for removing hexavalent chromium in a solution by marine Aspergillus niger. The steps are as follows: inoculate the marine Aspergillus niger strain on PDA medium, keep the temperature at 25-30°C, and collect spores; the preservation number of the marine Aspergillus niger strain is CCTCC NO: M2010132; inoculate the spores into the preparation medium, and keep the temperature in the shaker at 20-30°C to obtain Aspergillus niger mycelium balls; age the Aspergillus niger mycelium balls in water to obtain biological water treatment agents; put biological water treatment agents into The removal of hexavalent chromium is completed in the hexavalent chromium solution. The amount of biological water treatment agent added is 50g / L-200g / L, and the reduction efficiency of this method can reach 70-100%. At the same time, it can also absorb chromium ions in the solution, thereby reducing the total chromium content in the solution, and can be reused.

Description

technical field [0001] The invention relates to the technical field of chromium-containing wastewater treatment, in particular to a method for removing hexavalent chromium in a solution by marine Aspergillus niger. Background technique [0002] Hexavalent chromium is a key pollutant for prevention and control during the "Twelfth Five-Year Plan" period in my country. Among the types of heavy metal pollutants, chromium pollution ranks second. In 2010, the emission of hexavalent chromium exceeded 95 tons. At present, my country's annual output of chromium and its compounds exceeds 400,000 tons, accounting for more than 35% of the global output. [0003] Chromium generally exists in trivalent and hexavalent forms in the environment. Among them, hexavalent chromium has high activity and exists in a dissolved state in water, and has very strong toxicity. Moreover, hexavalent chromium is easily absorbed by the human body, and long-term, short-term exposure or inhalation has the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/14C12R1/685C02F101/22
Inventor 张其磊姚善泾白冬梅陆涛
Owner ZHEJIANG UNIV
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