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LAMP detection primer group for identifying pinellia ternata and adulterants thereof and kit and application thereof

A detection kit and detection primer technology, applied in the field of nucleic acid molecule identification of medicinal plants, can solve the problems of poor specificity of detection methods, inability to apply basic detection, low sensitivity, etc., achieve high specificity, save personnel and money costs, Sensitive effect

Inactive Publication Date: 2020-03-17
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention provides a LAMP detection primer set, kit and application thereof for identifying pinellia and its counterfeit products, which solves the problem of the long period required for the identification method of pinellia and the poor specificity of the detection method in the prior art. , low sensitivity, and cannot be applied to grassroots detection, etc.

Method used

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  • LAMP detection primer group for identifying pinellia ternata and adulterants thereof and kit and application thereof
  • LAMP detection primer group for identifying pinellia ternata and adulterants thereof and kit and application thereof
  • LAMP detection primer group for identifying pinellia ternata and adulterants thereof and kit and application thereof

Examples

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Effect test

Embodiment 1

[0042] The present embodiment provides a LAMP detection primer set for identifying pinellia and its counterfeit products, the sequence of the primer set is as follows:

[0043] ITS2-F3: the sequence is shown in SEQ ID NO: 1;

[0044] ITS2-B3: the sequence is shown in SEQ ID NO: 2;

[0045] ITS2-FIP: the sequence is shown in SEQ ID NO: 3;

[0046] ITS2-BIP: the sequence is shown in SEQ ID NO: 4;

[0047] ITS2-LF: the sequence is shown in SEQ ID NO: 5;

[0048] ITS2-LB: The sequence is shown in SEQ ID NO:6.

[0049] The embodiment of the present invention also provides a detection method for identifying pinellia and its counterfeit products, using the above-mentioned LAMP detection primer set to amplify the DNA of pinellia and its counterfeit products.

[0050] The LAMP identification primers and identification methods described in this example, compared with the original plant morphological identification, do not need to rely on the professional knowledge experience of the ap...

Embodiment 2

[0054] This example provides an application of the LAMP detection primer set described in Example 1 in the preparation of reagents for identifying pinellia and its counterfeit products.

Embodiment 3

[0056] This embodiment provides a detection reagent for identifying pinellia and its counterfeit products, including the LAMP detection primer set described in the above-mentioned embodiment 1.

[0057] Preferably, the detection reagent also includes dNTPs, betaine, MnCl 2 , 10×Bst buffer, calcein, BstDNA polymerase.

[0058] As a preference: the 10×ThermoPol buffer contains 20mM Tris-HCl (pH 8.8), 10mM KCl, 2mM MgSO 4 , 10mM (NH 4 ) 2 SO 4 , 0.1% Triton X-100.

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Abstract

The invention discloses a LAMP detection primer group for identifying pinellia ternate and adulterants thereof and a kit and an application thereof. A LAMP detection method is used for identifying pinellia ternata and the adulterants of pinellia ternata for the first time, the sensitivity is high, the specificity is high, the selected ITS2 sequence has good species identification capacity, and only authentic pinellia ternata can be subjected to an amplification reaction. The identification range is not limited to pinellia pedatisecta, pinellia cordata and rhizoma typhonii flagelliformis in thepatent, and other plants such as pedate pinellia, typhonium giganteum, pinellia cordata, Arisaema erubescens and the like can be identified.

Description

technical field [0001] The invention belongs to the field of identification of nucleic acid molecules of medicinal plants, and relates to rapid and accurate identification of medicinal plants Pinellia and its easily mixed products through loop isothermal amplification (LAMP) technology, and specifically relates to a method for identifying Pinellia and its mixed products LAMP detection primer set, kit and application of products. Background technique [0002] The shape of pinellia and its close genus such as pinellia palmatum, dripping pearl, and pinellia shui are very similar, especially the tubers of pinellia used as medicine. It is difficult to identify accurately and quickly by traditional morphological identification methods. Therefore, it is often used in the market. There are other plant tubers or fake and inferior products that are easy to mix up and pretend to be pinellia. At present, there are mainly three types of identification methods for pinellia chinensis. One...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6844C12N15/11
CPCC12Q1/6895C12Q1/6844C12Q2600/13C12Q2531/119
Inventor 王姝涵姚如永刘相萍隋爱华韩亚斐周泉刘加秀刘晓风
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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