40 results about "Pinellia pedatisecta" patented technology

The invention discloses a PPA-linker-thanatin fusion protein of which the amino acid sequence is as shown in SEQ ID NO: 2. The invention also discloses a preparation method of the PPA-linker-thanatin fusion protein, comprising the following steps of: acquiring target genes of recombinant pinellia pedatisecta schott agglutinin and thanatin by a two-step PCR (Polymerase Chain Reaction) method, cloning the target genes onto an E.coli expression vector pET-28a-c(+), and acquiring the expressed protein by IPTG (isopropyl thiogalactoside) induction, wherein the expressed protein is the PPA-linker-thanatin fusion protein, namely the expression product of the pinellia pedatisecta schott agglutinin-thanatin. The expression product of the pinellia pedatisecta schott agglutinin-thanatin has biological activity and has inhibiting effect on the growth of tumor cells.

The invention discloses pills for treating glaucoma and a preparation method, and belongs to the field of Chinese medicaments. Active ingredients of the pills consist of the following raw materials in parts by weight: 100 to 130 parts of lotus leaf, 90 to 120 parts of cassia seed, 75 to 95 parts of double hook, 70 to 90 parts of self-heal, 65 to 85 parts of pinellia tuber, 60 to 80 parts of motherwort fruit, 55 to 75 parts of rhizoma alismatis, 50 to 70 parts of white chrysanthemum, 45 to 65 parts of pyrrosia, 40 to 60 parts of mother of pearl, 35 to 55 parts of ligusticum, 35 to 50 parts of plantain seed, 30 to 45 parts of Baikal skullcap root, 30 to 40 parts of pipewort, 25 to 40 parts of areca nut, 20 to 35 parts of indigowoad leaf, 15 to 30 parts of rhizoma cyperi, 10 to 25 parts of fructus evodiae, 5 to 20 parts of pinellia pedatisecta schott, and 5 to 15 parts of bat dung. The formula is fine; according to the principles of liver and pulmonary tuberculosis heat and phlegm-damp function injury for initiating the glaucoma, medicaments for relieving liver heat and tonifying lung and qi are taken as monarchs, medicaments for clearing heat and eliminating phlegm are taken as ministers, and medicaments for removing damp and tonifying deficiency are taken as assistants and guides, so that the oxidation resistance of the body is improved, and the endocrine system is regulated; and through clinical verification, the pills have a good absorbing effect and high cure rate for various glaucoma diseases.

The invention relates to the technical field of chemical analysis and detection, in particular to a method for identifying whether pinellia is doped with Nanxing sativa: through liquid chromatography-mass spectrometer, specific detection ion pairs are used to detect the specific protein of Nanxing sativa. It can greatly improve the quality control level of Pinellia medicinal materials, and has a good guiding effect on the quality control of Pinellia decoction pieces and preparations containing Pinellia.

The invention belongs to the preparation of a novel gene, and more particularly relates to an aphid-resistant gene (PPA) and a preparation thereof and a protein using the aphid-resistant gene for coding. The 5' end of TCTAGAATGGCCTCCAAGCTCCTCCTC and the 3' end of CCCGGGC TACGCGGCAATTGGGCGCTT are taken as specific primers, a pinellia pedatisecta schott cDNA is taken as a template and a PCR amplification method is adopted so as to obtain the aphid-resistant gene (PPA); and the aphid-deadly protein is coded by the aphid-resistant gene (PPA). The invention solves the problem that the existing agglutinin gene with insect-resistant activity can not thoroughly inhibit the development and reproduction of insects, has aphid-deadly effect, obtains a full-length PPA gene and a plant expressed carrier constructed therefrom and lays foundation for further transforming the full-length PPA gene and the plant expressed carrier constructed therefrom into important crops such as cotton, wheat and rice to culture homoptera-insect-resistant transgenic plants; and can greatly reduce the use level of agricultural chemicals, achieve the aim of environmental protection and have great economic benefit andapplication value.

The invention relates to a medicinal extract for the treatment of the idiopathic facial palsy disease and a preparing method thereof. Drugs which can absorb clots and eliminate sputum, clear away heat and toxic materials and excite nerve are added on the basis of dispelling pathogenic wind and expelling evil factors from the muscles, adjusting ying and wei and strengthening the body resistance and eliminating pathogenic factors. The invention adopts the drugs calculated according to the proportion by weight: root kudzuvine 120 to 210 g, cassia twig 40 to 70 g, root of herbaceous peony 40 to 70 g, baikal skullcap root 40 to 70 g, human hair 40 to 70 g, frankincense 20 to 35 g, myrrh 20 to 35 g, dragon's blood resin 20 to 35 g, seed of nuxvomica poison nut 60 to 90 g, castor seed 60 to 90 g, pinellia pedatisecta schoot 35 to 45 g, gastrodia tuber 40 to 70 g, dried body of scorpion 40 to 70 g and fired pangolin scales 20 to 35 g; and supplementary materials as follows: litharge 952 to 1597 g, powder spice 40 to 70 g, bean oil 1270 to 2130 ml and sesame oil 635 to 1065 ml, the drugs and the supplementary materials are decocted into the medicinal extract, and two pastes are simultaneously pasted on the shangguan point and the mandible point of a patient. With remarkable therapeutic effects, high curative ratio, less side effects, and short effect time, the medicinal extract is low in cost and is applicable to the old and the young men with less contraindication and the normal working is not influenced due to the convenient usage. The preparing method is simple and easy to be mastered and operated.

The invention discloses a method for discriminating adulteration of fake Pinellia pedatisecta in pinellia tuber. The method comprises the following steps: 1, preparing a sample solution; 2, detecting the sample solution; and 3, discriminating the solution. A test result proves that the method allows the peaks of pinellia tuber and Pinellia pedatisecta to be effectively separated through screening an extraction technology and a high performance liquid chromatography technology, and an appropriate characteristic discriminating peak for discriminating Pinellia pedatisecta from pinellia tuber is found, so the method can be used for discriminating pinellia tuber and Pinellia pedatisecta or processing products thereof, and also can be used for discriminating adulteration of Pinellia pedatisecta or a processing product in pinellia tuber. The method is stable, reliable, simple and fast, can effectively monitor the quality of the pinellia tuber medicinal material, guarantees the curative effect and the medicine use safety of the pinellia tuber, and has wide market application prospect.

The invention discloses buccal granules for chronic pharyngolaryngitis and a preparation method, and belongs to the field of Chinese medicaments. The active ingredients of the buccal granules comprise the following raw materials in parts by weight: 50 to 70 parts of dwarf lilyturf tuber, 50 to 65 parts of rehmannia root, 45 to 60 parts of prepared rehmannia root, 45 to 60 parts of root-bark of tree peony, 40 to 55 parts of platycodon grandiflorum, 40 to 50 parts of licorice, 40 to 50 parts of root of straight ladybell, 35 to 45 parts of blackberry lily, 35 to 45 parts of white peony root, 30 to 40 parts of red peony root, 25 to 40 parts of dendrobium, 20 to 30 parts of glossy privet fruit, 20 to 30 parts of pinellia pedatisecta schott, 15 to 30 parts of loquat leaf, 15 to 25 parts of typha, 10 to 25 parts of cicada slough, 10 to 20 parts of prelude, 10 to 20 parts of dark plum, 10 to 20 parts of cynanchum atratum, and 5 to 18 parts of almond. The medicinal materials are selected according to the principle of monarch, minister, assistant and guide; diseases are treated by proper medicaments; and the buccal granules have the effects of nourishing yin, clearing heat, moistening lung and clearing away the heart fire, have a good absorption effect and a remarkable treatment effect, do not have toxic or side effect or clinical adverse response, and are high in cure rate of the chronic pharyngolaryngitis by clinical verification.

The present invention discloses a metaplexis japonica essence qi supplementing and benefiting milky tea and a production method thereof. The milky tea consists of the following raw materials: metaplexis japonica, pine nuts, radix glehniae, walnut kernels, saarcodon imbricatum, radish seeds, haws, fructus aurantii immaturus, fructus galangae, milky tea powder, spices, etc. The milky tea is prepared by material selecting, drying, boiling, milling, mixing, sterilizing, and packaging. The active ingredients in the traditional Chinese medicines are boiled into an extract, the extract is prepared into powder, the powder is combined with milky tea, and the nutrients in the traditional Chinese medicines are effectively retained. Through a scientific ratio, the products have efficacies of nourishing body, supplementing body without dryness, being reasonable in nutrition structure, and enhancing human body constitution. The milky tea is health-care, can supplement essence qi, and is pure natural, non-polluting, fragrant and mellow in tastes, free of toxic or side effects, etc.

Disclosed is a topical drug for treating breast cancer and preparation method thereof. The topical drug comprises the following ingredients in parts by weight: 5-10 parts of Astragalus, 3-5 parts of Oldenlandia diffusa, 3-5 parts of dandelion, 5-10 parts of Lycoris radiate, 5-8 parts of Carapax trionycis, 3-5 parts of Pinellia pedatisecta, 2-3 parts of Acorus calamus, 3-5 parts of Ferula sinkiangensis, 3-5 parts of doubleteeth pubescent angelica root, 4-6 parts of Cryptotaenia japonica Hassk, 3-5 parts of Whitmania pigra Whitman, 5-8 parts of Wedelia chinensis, 6-8 parts of Echinacea purpurea, 4-6 parts of Folium Eriobotryae, 3-5 parts of Sedum sarmentosum, 5-10 parts of Angelicae sinensis, 2-5 parts of asparagus, 2-3 parts of scorpio, 5-8 parts of Rubia cordifolia L., 2-3 parts of alfalfa extract, 3-5 parts of extract of Jew's ear from mulberry trees, 5-7 parts of myrrh, 3-6 parts of nutgrass galingale rhizome and 3-5 parts of pericarpium citri reticulatae viride.