Pinellia pedatisecta trypsin inhibitor gene, protein encoded thereby, and anti-insect application thereof

Abstract

The invention discloses a pinellia pedatisecta trypsin inhibitor gene, a protein encoded thereby, and an anti-insect application thereof, wherein the nucleotide sequence table of the gene is represented as the SEQ ID No.1; during culturing an insect-resistant transgenic plant, firstly a recombinant expression vector containing the gene represented as the SEQ ID No.1 is constructed; then a transformant is constructed by means of the recombinant expression vector; finally the transformant is used for converting a target plant; positive plants then are screened; and through three generations of screening, a homozygous transgenic plant, having insect resistance relative to normal plant, can be produced. In the invention, by means of large quantity of scientific research, it is proved that thegene has significant improvement effect on insect resistance of plants and has a tight relationship with the insect resistance of plants. On the basis of the fact, the transgenic plant having high insect resistance can be prepared through a gene engineering method in the prior art. The gene has great practical significance for reducing use amount of pesticides, preventing and treating aphid and increasing planting efficiency and yield of the crops.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the preparation of pinellia palmata protease inhibitor gene, the encoded protein and its application in insect resistance. Background technique [0002] Aphids are the fastest-growing insects. They are widely harmful to crops on the earth, and they are difficult to control because they have developed a variety of self-protection defense methods. Some species can secrete plant hormones, making plants form gall nests; some secrete a A layer of wax covering the body surface makes it difficult for drugs to penetrate; some release a strong smell of mustard oil to scare away natural enemies. Aphids are also the media of many diseases in agriculture, and the secretion of cotton aphids is an important allergen of human respiratory tract, which seriously damages the living environment in urban and rural areas. Pinellia palmatum ( Pinellia pedatisecta ) is a Chinese herbal me...

AI Technical Summary

Problems solved by technology

[0003] (1) There are few aphid resistance genes cloned at present

Three major classes of protease inhibitors have been found in plants: serine, mercapto and metalloprotease inhibitors. The PPTI protein of the present invention belongs to the first class and serine protease inhibitors. Serine protease inhibitors have also been found to have at least 6 There is a family, but only some members have strong resistance function. In addition to the above, there are also protease inhibitors of bean and potato. Transgenic plants with toxic protease inhibitor genes paired with constitutive promoters still have low protease inhibitor expression levels and generally do not reach the lethal dose of aphids

[0006] In summary, the basic research on aphid resistance from the current research status at home and abroad, there are problems include: first, there are few researches on the resistance genes of crops to aphids, and there are few broad-spectrum single-antibiotic genes available; second, broad-spectrum Most of the plant lectins contain sugar chains, and it is difficult to express the correct sugar chains by genetic engineering methods; third, the existing protease inhibitor genes with constitutive promoters of transgenic plants generally do not reach the lethal dose, and the development is effective. The protease inhibitor gene of pollution-free control of aphids is of great significance

Images