Method for eliminating viruses of pinellia pedatisecta
A technology for removing the palm and leaves of Pinellia sinensis, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of decreased yield and quality, dwarf plants, slow growth, etc., and achieve the effect of improving yield and quality.
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Embodiment 1
[0043] The method for removing Pinellia palmata virus comprises the following process steps:
[0044] a. Low temperature treatment
[0045] The roots of Pinellia palmatum overwintering under natural conditions shall be covered with 3-5 cm of soil. When the ground temperature at the root is -2°C-3°C, dig out the root soil and take the bud tips that have just germinated;
[0046] b. High temperature treatment
[0047] Rinse the removed bud tips, place them in a petri dish covered with wet filter paper, and place them in an incubator for 15 minutes at a temperature of 50°C at high temperature;
[0048] c. Disinfection treatment
[0049] Rinse the high-temperature-treated bud tips with soapy water and sterilize them on an ultra-clean workbench;
[0050] d, cut bulbil culture:
[0051] Put the sterilized bud tip on the ultra-clean workbench, under the binocular solid dissecting microscope, and dissect the bud tip growth point with a size of 0.3-0.5 mm, inoculate it into the ind...
Embodiment 2
[0074] The difference between this embodiment and embodiment 1 is:
[0075] The length of bud tip in the described step a is 0.3cm.
[0076] The sterilization treatment in the step c is to use alcohol solution to sterilize the bud tip surface for 10 seconds, then sterilize it with 0.1% mercuric chloride solution for 1 minute, rinse it with sterile water for 3 times, and suck the bud body with sterilized filter paper Moisture on the surface in preparation for cutting bulbils.
[0077] The culture conditions in the step d are: temperature 25°C, light 3000Lx.
[0078] All the other contents are the same as in Example 1.
Embodiment 3
[0080] The difference between this embodiment and embodiment 1 is:
[0081] The length of bud tip in the described step a is 0.5cm.
[0082] The sterilization treatment in the step c is to use alcohol solution to sterilize the bud tip surface for 20 seconds, then sterilize it with 0.1% mercuric chloride solution for 3 minutes, wash it with sterile water for 5 times, and suck the bud body with sterile filter paper Moisture on the surface in preparation for cutting bulbils.
[0083] The culture conditions in the step d are: temperature 28°C, light 2000Lx.
[0084] All the other contents are the same as in Example 1.
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