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Method for discriminating adulteration of Pinellia pedatisecta in pinellia tuber

A technology of tiger palm and pinellia, applied in the field of identification of traditional Chinese medicinal materials, can solve problems such as harming the interests of Pinellia planters and production enterprises of Pinellia decoction pieces, affecting the quality, curative effect and medication safety of Pinellia sinensis, and difficulty in identification, etc. Guaranteed efficacy and drug safety, broad market application prospects, stable and reliable results

Active Publication Date: 2017-02-15
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After processing, the appearance of tiger palm is very similar to Pinellia, and it is difficult to identify. Unscrupulous traders often add this medicinal material to Pinellia to illegally seek profits, which seriously damages the interests of Pinellia growers and Pinellia decoction pieces manufacturers, and also seriously Affect the quality, curative effect and drug safety of pinellia

Method used

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  • Method for discriminating adulteration of Pinellia pedatisecta in pinellia tuber
  • Method for discriminating adulteration of Pinellia pedatisecta in pinellia tuber
  • Method for discriminating adulteration of Pinellia pedatisecta in pinellia tuber

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 The inventive method distinguishes pinellia and tiger's palm

[0047] 1. Preparation of the test solution

[0048] Take 1g of pinellia or tiger palm medicinal powder, add 20mL of water, ultrasonically extract for more than 30min, filter or centrifuge, take 10mL of clarified extract, add 0.1mL of phosphoric acid, extract 4 times with ethyl acetate, 20-30mL each time, recover ethyl acetate ester, the residue was dissolved in 5 mL of water, and passed through a microporous membrane to obtain the product.

[0049] 2. Chromatographic conditions

[0050] Mobile phase: A (acetonitrile)-B (0.1% phosphoric acid) (3:97)

[0051] Chromatographic column: Agilent ZORBAX Eclipse Plus C18 liquid chromatography column (4.6×250mm, 5μm);

[0052] Flow rate: 0.8mL / min;

[0053] Detection wavelength: 210nm;

[0054] Column temperature: 40°C;

[0055] Injection volume: 20 μL.

[0056] 3. Results

[0057] (1) The chromatograms of 10 Pinellia samples from different source...

Embodiment 2

[0064] The process screening of embodiment 2 the inventive method

[0065] 1. Investigation of extraction method

[0066] (1) Ratio of material to liquid

[0067]Weigh 1g of tiger palm sample, the amount of extraction solvent water is 20mL, 30mL, 40mL, ultrasonic extraction, respectively take 10mL of clarified extract, add 0.1mL of acid, extract with ethyl acetate, recover solvent, dissolve in 5mL of water, pass through microporous filter Membrane, sample loaded. The results are shown in Table 1.

[0068] Table 1 Investigation of different material-to-liquid ratios

[0069] solid to liquid ratio 1:20 1:30 1:40 Peak area 174 107 75 Converted peak area 174 160 150

[0070] The results showed that the peak area was the highest when the ratio of solid to liquid was 1:20, and the extraction effect was the best.

[0071] (2) Investigation of extraction time

[0072] Weigh 1g of tiger palm sample, add 20mL of water, and extract by ultrasonic. Th...

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Abstract

The invention discloses a method for discriminating adulteration of fake Pinellia pedatisecta in pinellia tuber. The method comprises the following steps: 1, preparing a sample solution; 2, detecting the sample solution; and 3, discriminating the solution. A test result proves that the method allows the peaks of pinellia tuber and Pinellia pedatisecta to be effectively separated through screening an extraction technology and a high performance liquid chromatography technology, and an appropriate characteristic discriminating peak for discriminating Pinellia pedatisecta from pinellia tuber is found, so the method can be used for discriminating pinellia tuber and Pinellia pedatisecta or processing products thereof, and also can be used for discriminating adulteration of Pinellia pedatisecta or a processing product in pinellia tuber. The method is stable, reliable, simple and fast, can effectively monitor the quality of the pinellia tuber medicinal material, guarantees the curative effect and the medicine use safety of the pinellia tuber, and has wide market application prospect.

Description

technical field [0001] The invention relates to the field of identification of traditional Chinese medicinal materials, in particular to a method for identifying adulterated tiger palms in pinellia ternata. Background technique [0002] Pinellia is the dry tuber of Pinellia ternata (Thunb.) Breit, a plant of the genus Pinellia in the Araceae family. Excavated in summer and autumn, washed, removed the skin and fibrous roots, and dried in the sun. [Sex and Meridian Return] Pungent, warm; poisonous. Return spleen, stomach, lung meridian. [Functions and Indications] Drying dampness and resolving phlegm, lowering adverse flow and relieving vomiting, dispelling swelling and dissipating stagnation. For wet phlegm and cold phlegm, cough and dyspnea with excessive phlegm, dizziness and palpitation due to phlegm retention, wind-phlegm dizziness, syncope due to phlegm, headache, vomiting and regurgitation, fullness in the chest cavity, plume nucleus qi; external treatment of carbunc...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 李敏敬勇陈辉陶玲何金晓刘佳灵
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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