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Novel coronavirus neutralizing antibody qualitative detection kit based on colloidal gold double-antibody sandwich method

A detection kit, coronavirus technology, applied in the field of disease diagnosis, to achieve high sensitivity, increase labeling yield, and avoid crossover effects

Active Publication Date: 2022-04-12
浙江默乐生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In 2019, the outbreak of the new coronavirus has caused a global pandemic, and this epidemic has a long duration and repetition

Method used

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  • Novel coronavirus neutralizing antibody qualitative detection kit based on colloidal gold double-antibody sandwich method
  • Novel coronavirus neutralizing antibody qualitative detection kit based on colloidal gold double-antibody sandwich method
  • Novel coronavirus neutralizing antibody qualitative detection kit based on colloidal gold double-antibody sandwich method

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Experimental program
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Embodiment 1

[0029] 1 Preparation of colloidal gold

[0030] The prepared colloidal gold is a colloidal gold gold sol prepared by the trisodium citrate reduction method, and the particle diameter is 40 nm. Uniform in size, it can effectively improve the binding of colloidal gold and SARS-CoV-2 S-RBD protein.

[0031] (1) Prepare 2ml of 0.1g / ml aqueous solution of chloroauric acid, and store in the dark for future use.

[0032] (2) 10ml of 0.1g / ml trisodium citrate aqueous solution was prepared, and stored in the dark for future use.

[0033] (3) Add the volume of colloidal gold solution to be prepared into the beaker, such as: 1L water; then place the beaker on a magnetic stirrer and heat it to boiling.

[0034] (4) 1.53ml of chloroauric acid aqueous solution was added to the beaker while heating; then 0.24ml of trisodium citrate solution was added to the beaker containing chloroauric acid aqueous solution, and continued stirring for 5 minutes to obtain a colloid of 40nm Gold solution. ...

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Abstract

The invention relates to a novel coronavirus neutralizing antibody qualitative detection kit based on a colloidal gold double-antibody sandwich method, the novel coronavirus neutralizing antibody qualitative detection kit comprises a test strip and a sample diluent, the test strip comprises a sheet material, nano colloidal gold labeled novel coronavirus S-RBD protein, a sample pad, a detection line and a quality control line, and the novel coronavirus neutralizing antibody qualitative detection kit is characterized in that the sample diluent comprises a sample diluent and a sample diluent, the nano colloidal gold labeled novel coronavirus S-RBD protein contains BSA (bovine serum albumin) and PEG (polyethylene glycol), the sample diluent comprises a surfactant, a Tris buffer solution and bovine serum albumin, and the surfactant is selected from one or a combination of more of laurinol polyethylene oxide (9) ether, laurinol polyoxyethylene ether, fatty alcohol epoxy ether and oleyl alcohol polyoxyethylene (10) ether. By adopting the technical scheme provided by the invention, the stability of the S-RBD colloidal gold marker can be improved, and the sensitivity and specificity of the kit can be improved.

Description

technical field [0001] The invention relates to the field of disease diagnosis, in particular to a novel coronavirus neutralizing antibody qualitative detection kit based on a colloidal gold double-antibody sandwich method and an application thereof. Background technique [0002] Colloidal gold labeling technology is a labeling method developed in the 1980s following the three major labeling technologies of fluorescein, radioisotopes and enzymes. In 1971, Faulk and Taylor introduced colloidal gold into immunobiochemistry. Since then, this labeling method has been widely used in the fields of drug detection, biomedical detection, pesticide and veterinary drug detection, and clinical disease detection. At present, there are hundreds of colloidal gold immunochromatography kits commercialized at home and abroad, such as: early pregnancy kits, drug detection kits, fecal occult blood detection kits, infectious disease detection kits Wait. [0003] Colloidal gold labeling technol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/569G01N33/558G01N33/543
CPCY02A50/30
Inventor 卜令杰李扬霞陈超
Owner 浙江默乐生物科技有限公司
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