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Method for identifying whether pinelliapedatisecta exists in pinelliaternata

A technology of the Southern Star of Tiger Palm and Pinellia, which is applied in the field of identifying whether Pinellia is mixed with Southern Star of Tiger Palm, and achieves the effects of improving the quality control level, strong specificity, and easy to popularize and use

Active Publication Date: 2019-03-29
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems in the prior art that there is no good identification method for Pinellia chinensis, this application discloses a method for identifying whether Pinellia is adulterated with counterfeit S. method

Method used

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  • Method for identifying whether pinelliapedatisecta exists in pinelliaternata
  • Method for identifying whether pinelliapedatisecta exists in pinelliaternata
  • Method for identifying whether pinelliapedatisecta exists in pinelliaternata

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Experimental program
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Effect test

preparation example Construction

[0027] Preparation of relevant solutions in the test

[0028] Denaturation buffer: Weigh 573.1g of guanidine hydrochloride, 121.1g of tris, 0.734g of ethylenediaminetetraacetic acid, add water to dissolve, add concentrated hydrochloric acid to adjust the pH to 8.0, add water to dilute to 1L, and shake well.

[0029] DTT solution: Weigh an appropriate amount of DTT (dithiothreitol), dissolve it in water, and get it (concentration is 0.50M);

[0030] IAA solution: Weigh an appropriate amount of IAA (iodoacetamide), dissolve it in water, and get it (concentration is 0.55M, ready to use);

[0031] Ammonium bicarbonate solution: Weigh an appropriate amount of ammonium bicarbonate and dissolve it in water to obtain (concentration: 1.0%);

[0032] Acetic acid solution: Accurately measure an appropriate amount of acetic acid and dilute it with water to get it (concentration is 0.5%);

[0033] Bovine trypsin solution: Weigh an appropriate amount of bovine trypsin, dissolve it in acet...

Embodiment 1

[0035] 1 Instruments, reagents and samples

[0036] Instruments: Waters Quattro Premier XE high performance liquid chromatography-mass spectrometry; Sartorius CP225D electronic balance.

[0037] Reagents: trypsin (manufactured by Sigma, batch number: SLBG6452V); dithiothreitol (DTT), iodoacetamide (IAA), ammonium bicarbonate, and acetic acid were all of analytical grade.

[0038] Sample: pinellia herbal medicine (collected in the market). Pinellia control medicinal materials (provided by the China Inspection Institute, batch number: 121272-201404 and 121272-201605).

[0039] Liquid phase and mass spectrometry conditions

[0040] Liquid phase conditions: the column is ACQUITY UPLC ® BEH C 18(2.1×100mm, 1.7μm), column temperature 35°C, flow rate 0.3mL / min, mobile phase A is 0.1% formic acid solution, B is 0.1% formic acid acetonitrile solution, gradient elution is performed, see the table below.

[0041] Table 1 Gradient elution program

[0042]

[0043] Mass spectrome...

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PUM

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Abstract

The invention relates to the technical field of chemical analysis and detection, and particularly relates to a method for identifying whether pinelliapedatisecta exists in pinelliaternata. The methodcomprises the following step of detecting the specific protein of the pinelliapedatisectaby liquid chromatography-mass spectrometry and using specific detection ions. The invention can greatly improvethe quality control level of the pinelliaternata, and has a good guiding effect on the quality control of the pinelliaternata and the preparations containing the pinelliaternata. The method can alsobe used for medicinal material quality control of the pinelliapedatisecta.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for identifying whether Pinellia pinellia is doped with Southern Tiger Palm. Background technique [0002] Pinellia is a valuable and commonly used Chinese herbal medicine, but the adulteration of this product is very serious. Most of the Pinellia herbs circulating in the market are mixed with other adulterated products, and even some batches of Pinellia samples are all adulterated. . Its common adulterated products include Tiannanxing, Huzhang Nanxing and Shui Pinellia, etc., especially the smaller Huzhang Nanxing because it has a very similar shape to the genuine Pinellia, and it is less difficult to plant, has a high yield, and the price is only It is one-fifth of Pinellia, so this variety has become the main source of counterfeit products of Pinellia. Tiger Palm Southern Star and Pinellia have significantly different functions and indication...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8872
Inventor 王玉团石峰杭宝建咸瑞卿梁翠荣巩丽萍
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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