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Method for detecting adulteration of pinellia pedatisecta Schott in pinellia ternata

A detection method, the technology of Nanxing Huzhang, which is applied in the field of detection of adulterated Nanxing Huzhang in pinellia, can solve the problems of difficult identification, insufficient rigor, and inaccurate identification, and achieve good precision, strong specificity, and repeatability Good results

Pending Publication Date: 2019-08-06
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to seek profit, there are many traders who processed tiger palms and then screened and polished them. The original sub-tubers have no obvious characteristics and are very similar to pinellia, making it difficult to identify them. If they are processed into decoction pieces, they can hardly be identified by their characteristics.
At present, there are still many problems in the identification of the characteristics of Pinellia and Rhizoma chinensis. It is traditionally believed that Pinellia is spherical or oval, and generally has no sub-tubers. However, there are cultivation variations in cultivated Pinellia. For tubers, this has caused the identification of Pinellia and Tiger Palm by the presence or absence of sub-tubers, and there is a problem of inaccurate identification. Some judge Pinellia and Tiger Palm by the number of sub-tubers (more than 3 are judged as Tiger Palm ), this method is still not rigorous enough to achieve the purpose of accurate identification
[0006] Chinese patent CN106680397A discloses a HPLC detection method using water ryotropic acid as a detection index to distinguish pinellia and Rhizoma chinensis. Consistent chromatographic peaks lead to "false positives" and interfere with the judgment of experimental results

Method used

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  • Method for detecting adulteration of pinellia pedatisecta Schott in pinellia ternata
  • Method for detecting adulteration of pinellia pedatisecta Schott in pinellia ternata
  • Method for detecting adulteration of pinellia pedatisecta Schott in pinellia ternata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 The detection method of the present invention is used for the detection of adulterated tiger palms in pinellia

[0039] 1) Preparation of reference solution

[0040] Take an appropriate amount of the reference substance of lysopogonic acid in water, accurately weigh it, add water to make a solution containing 0.25 μg per 1 ml, and use it as the reference substance solution.

[0041] 2) Preparation of the test solution

[0042] Take about 1 g of pinellia sample powder (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered conical flask, add 20 ml of water accurately, weigh it, and ultrasonically treat it (power 250W, frequency 40kHz) for 45 minutes, let it cool, and then Weigh the weight, make up the lost weight with water, filter, accurately measure 10ml of the filtrate to a 50ml centrifuge tube, add 0.1ml of phosphoric acid, shake well, add 20ml of ethyl acetate, shake well, and centrifuge (5000 rpm Turn), separate the ethyl acetate s...

Embodiment 2

[0048] Embodiment 2 The detection method of the present invention is used for the detection of adulterated tiger palms in pinellia

[0049] 1) Preparation of reference solution

[0050] Take an appropriate amount of the reference substance of lysopogonic acid in water, accurately weigh it, add water to make a solution containing 0.25 μg per 1 ml, and use it as the reference substance solution.

[0051] 2) Preparation of the test solution

[0052] Take about 1 g of pinellia sample powder (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered conical flask, add 20 ml of water accurately, weigh it, and ultrasonically treat it (power 250W, frequency 40kHz) for 45 minutes, let it cool, and then Weigh the weight, make up the lost weight with water, filter, accurately measure 10ml of the filtrate to a 50ml centrifuge tube, add 0.1ml of phosphoric acid, shake well, add 20ml of ethyl acetate, shake well, and centrifuge (5000 rpm Turn), separate the ethyl acetate s...

experiment example 1

[0060] Experimental example 1 Comparison of detection effects of different mobile phases

[0061] Except for the mobile phase, the rest of the operations are the same as in Example 1, respectively detect the chromatograms of philopogon acid, Pinellia radix and Nanxing Xinghuo, and observe whether the HPLC chromatogram of the Pinellia sample appears to be consistent with the retention time of the lysotropic acid reference substance chromatographic peaks. The chromatographic conditions are as follows:

[0062] A1: Acetonitrile-0.01% phosphoric acid solution (3:97), flow rate 0.8ml / min

[0063] A2: Acetonitrile-0.01% phosphoric acid solution (1:99), flow rate 0.8ml / min

[0064] A3: Acetonitrile-0.1% phosphoric acid solution (1:99), flow rate 0.8ml / min

[0065] See the test results figure 1 , figure 2 and image 3 .

[0066] The results show that: using A1: acetonitrile-0.01% phosphoric acid solution (3:97) mobile phase system, the separation degree of lysoporic acid in Hu...

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Abstract

The invention discloses a method for detecting adulteration of pinellia pedatisecta Schott in pinellia ternate. The method adopts an efficient liquid chromatography for detection. The method overcomesthe problem that experimental result judgment is affected by chromatographic peaks which are consistent with retention time of a triglochinic acid reference product in an HPLC map of partial pinelliaternata sample. The detecting method has the advantages of high specificity, good precision, good repeatability and high precision, and can be used for identifying adulteration of pinellia pedatisecta Schott in pinellia ternata precisely.

Description

technical field [0001] The invention specifically relates to a method for detecting the adulterated southern star of tiger palm in Pinellia chinensis. Background technique [0002] Pinellia is the dry tuber of Pinellia ternata (Thunb.) Breit., a plant of the family Araceae. It has the effects of drying dampness and resolving phlegm, reducing adverse reactions and relieving vomiting, and eliminating mass. For wet phlegm and cold phlegm, cough and dyspnea with excessive phlegm, dizziness and palpitation due to phlegm retention, wind-phlegm dizziness, syncope due to phlegm, headache, vomiting and regurgitation, fullness in the chest cavity, plume nucleus qi; external treatment of carbuncle and swollen phlegm nucleus. [0003] Pedatisecta Schott is the dry tuber of Pinelliae Pedatisecta Schott, a plant of the genus Pinellia in the family Araceae. [0004] There are many counterfeit products of Pinellia, mainly Tiger Palm, Araceae, and Pinellia of the family Araceae. After proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李敏敬勇陈辉刘佳灵赖月月
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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