Application of jasmonic acid-isoleucine hydroxylase encoding gene segment and silent vector thereof in increasing yield of potatoes

A gene fragment, potato technology, applied in the field of genetic engineering, can solve the problems of large labor and material costs, limited increase in yield, etc.

Active Publication Date: 2020-04-03
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods all need to input a large amount of labor and material costs, and the range of increasing production is limited.

Method used

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  • Application of jasmonic acid-isoleucine hydroxylase encoding gene segment and silent vector thereof in increasing yield of potatoes
  • Application of jasmonic acid-isoleucine hydroxylase encoding gene segment and silent vector thereof in increasing yield of potatoes
  • Application of jasmonic acid-isoleucine hydroxylase encoding gene segment and silent vector thereof in increasing yield of potatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] StCYP94B3-1, StCYP94B3-2 and StCYP94B3-3 genes were simultaneously silenced

[0045] The StCYP94B3-1, StCYP94B3-2 and StCYP94B3-3 genes in the potato were compared, and the 283bp with higher nucleotide sequence similarity in the cDNA sequence was selected ( figure 1 The sequence framed in the middle frame, shown in SEQ ID NO.1), is used to construct the gene silencing vector: use the primer pair shown in SEQ ID NO.11~12 to amplify from potato leaf cDNA by RT-PCR with high-fidelity enzyme After the positive clones were selected and verified by sequencing, they were cloned into the pHELLSGATE12 vector by the Gateway LR reaction, and then transformed into Agrobacterium GV3101 after sequencing verification.

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Abstract

The invention provides application of a gene segment of a jasmonic acid-isoleucine hydroxylase encoding gene StCYP94B3-like and a silent vector of the gene segment in increasing the yield of potatoesand relates to the technical field of gene engineering. The cDNA (complementary desoxyribonucleic acid) sequence of the gene segment of StCYP94B3-like is shown in SEQ ID NO.1 in the description. Afterthe RNAi (ribonucleic acid interfere) silent vector is constructed by using the StCYP94B3-like gene segment and transformation of the silent vector into potatoes, the content of jasmonic acid-isoleucine (JA-Ile) can be remarkably increased, the content of jasmonic acid (JA) is not largely affected, and furthermore, the yield of the potatoes can be increased.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the application of potato jasmonic acid-isoleucine hydroxylase coding gene fragment and its silencing vector in improving potato yield. Background technique [0002] Potato is the fourth largest food crop in the world after corn, rice and wheat. Potato tuber development is a complex comprehensive control process, which not only involves many environmental regulation factors, but also is related to the content and state of endogenous growth active substances. These environmental factors and endogenous hormones ultimately affect the expression of related genes, thereby regulating the occurrence and development of tubers. [0003] Among many environmental regulation factors, light, temperature, and medium sucrose concentration under in vitro culture conditions are the main factors affecting tuber formation. The study found that short-day sunshine promotes t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N15/82A01H5/00A01H6/82
CPCC12N9/0073C12N15/8218C12N15/8261C12Y114/13117
Inventor 王蕾杨飞汤金香展康徐尤先韩小女杨亚琼曹国艳何彩花
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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