A kind of ace inhibitory peptide using chrysanthemum yellow puffer fish skin as raw material and preparation method

A technology for inhibiting peptides and fish skins, applied in the field of ACE inhibitory peptides

Active Publication Date: 2021-04-09
FISHERIES RES INST OF FUJIAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are existing technologies for preparing ACE-inhibiting peptides from hazelnuts, yak milk, shellfish, wolfberry and other foods, but there is no technology for preparing ACE-inhibiting peptides from puffer fish, especially puffer fish skin as raw materials

Method used

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  • A kind of ace inhibitory peptide using chrysanthemum yellow puffer fish skin as raw material and preparation method
  • A kind of ace inhibitory peptide using chrysanthemum yellow puffer fish skin as raw material and preparation method
  • A kind of ace inhibitory peptide using chrysanthemum yellow puffer fish skin as raw material and preparation method

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Embodiment Construction

[0036] The present invention will be further described in conjunction with the accompanying drawings and specific embodiments.

[0037] This example provides an ACE-inhibiting peptide that uses chrysanthemum yellow oriental puffer fish skin as a raw material, the inhibitory peptide is an octapeptide, and the amino acid sequence is GPRGPQGP (Gly-Pro-Arg-Gly-Pro-Gln-Gly-Pro).

[0038] The octapeptide GPRGPQGP (Gly-Pro-Arg-Gly-Pro-Gln-Gly-Pro) has a molecular weight of 764.4 Da.

[0039] This embodiment also provides a method for preparing an ACE-inhibiting peptide using chrysanthemum yellow oriental puffer fish skin as a raw material, comprising the following steps:

[0040]S1: Preparation of puffer fish enzymatic hydrolyzate, after beating 100g puffer fish skin, add 300mL distilled water and stir to form protein solution, adjust pH value to 8.0 with NaOH, then add alkaline protease, the enzyme amount is 500U / g, Enzyme hydrolyze at 55°C for 10 hours, keep the pH value unchanged...

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Abstract

The invention discloses an ACE inhibitory peptide using chrysanthemum yellow oriental fugu skin as a raw material and a preparation method thereof, belonging to the technical field of ACE inhibitory peptides, and a method for preparing an ACE inhibitory peptide using chrysanthemum yellow oriental fugu skin as a raw material, comprising The steps are as follows: S1: preparation of puffer fish enzymatic hydrolyzate, S2: ultrafiltration separation of puffer fish enzymatic hydrolyzate, S3: separation and purification of polypeptide, S4: sequence identification of polypeptide, S5: synthesis of polypeptide and determination of activity. An ACE-inhibiting peptide that uses chrysanthemum yellow oriental puffer fish skin as a raw material, the inhibitory peptide is an octapeptide, and the amino acid sequence is GPRGPQGP (Gly‑Pro‑Arg‑Gly‑Pro‑Gln‑Gly‑Pro). The ACE-inhibiting peptide of the present invention uses chrysanthemum-yellow oriental puffer fish skin as a raw material and its preparation method. The octapeptide amino acid sequence is obtained by using chrysanthemum-yellow oriental puffer fish skin as a raw material, and the prepared polypeptide has ACE inhibitory activity.

Description

technical field [0001] The invention belongs to the technical field of ACE inhibitory peptides, and in particular relates to an ACE inhibitory peptide using chrysanthemum yellow oriental puffer fish skin as a raw material and a preparation method thereof. Background technique [0002] With the progress and development of society, people's living standards are gradually improving, and the proportion of fat and calories in the diet is gradually increasing. Coupled with the reduction in exercise, the incidence of cardiovascular diseases in my country has risen rapidly, of which hypertension is the main one. [0003] Pufferfish is a bottom fish in the warm temperate zone and tropical coastal waters. It inhabits the middle and lower layers of the ocean. A few species enter freshwater rivers. When encountering external danger, the whole body floats to the surface in a spherical shape, and at the same time, the small spines on the skin stand up. , for self-defense. [0004] Angiot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/16C12P21/06
CPCC07K7/06C12P21/06
Inventor 刘智禹苏永昌刘淑集陈贝乔琨许旻蔡水淋
Owner FISHERIES RES INST OF FUJIAN
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