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Method for inducing efficient attachment metamorphosis of Crassostrea hongkongensis

A technology for attaching metamorphosis and Hong Kong oysters, which is applied in the fields of climate change adaptation, fish farming, application, etc., can solve the problems of unsatisfactory larval attachment metamorphosis rate and low artificial seedling efficiency, and achieve the effect of increasing the attachment metamorphosis rate and shortening the time.

Active Publication Date: 2020-05-15
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

In recent years, in response to market requirements, Hong Kong oyster artificial seedling technology has been greatly developed. Compared with other oysters, the artificial seedling efficiency of Hong Kong oyster is still not high, mainly because the larval metamorphosis rate is not ideal. Technical bottleneck, the present invention specially designed a set of highly efficient technical methods for inducing metamorphosis

Method used

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Examples

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Embodiment 1

[0026] This embodiment is completed in the southeast test station of Donghai Island in Zhanjiang. Specifically, 10 culture ponds are used, and the total number of attached metamorphic water bodies reaches 200m 3 , the total number of treated larvae reached more than 250 million.

[0027] A method for inducing high-efficiency attachment metamorphosis of Hong Kong oysters, comprising the following steps:

[0028] (1) After the Hong Kong oyster larvae were cultured to the 13th day, about 50% of the larvae reached a size of 320 μm, and the salinity of the culture water was reduced by 2‰ every day until the salinity was adjusted to 10‰. At this time, the larva culture density was adjusted to about 2.5 / mL, and the cloud microalgae and concentrated chaetoceros were fed;

[0029] (2) Two days before the induction of attachment metamorphosis, start the preparation of the attachment metamorphosis pool: move the cleaned and sterilized attachment base into the attachment metamorphosis po...

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Abstract

The invention discloses a method for inducing efficient attachment metamorphosis of Crassostrea hongkongensis. The method comprises the following steps: (1) after Crassostrea hongkongensis larvae arecultured to a certain specification, starting to decrease salinity of a culture water body in each day, and carrying out conventional feeding management; (2) moving a cleaned and disinfected attachment medium into an attachment metamorphosis pond, filling the pond with water for the first time, carrying out draining, filling the pond with micron-level filtered seawater, adding EDTA and air heads;(3) after detecting that the ratio of eyespot larvae is over a half by virtue of a microscope, pouring the larvae into the pond, carrying out sieving and screening, transferring big larvae into the attachment metamorphosis pond in the step (2), uniformly scattering ZnCl2 induction liquid into the pond, and carrying out air inflation and uniform mixing; and (4) after the attachment metamorphosis ofthe eyespot larvae is finished, draining the water, injecting the micron-level filtered seawater into the pond again, carrying out decrement feeding in the first day, carrying out increment feeding in the second day, and carrying out doubled feeding management after 72h. The method is simple, convenient and feasible, is suitable for large-scale production, application and popularization and has obvious promotion effects to other oysters.

Description

technical field [0001] The invention relates to the technical field of shellfish seed breeding in marine agriculture, in particular to a method for inducing high-efficiency attachment and metamorphosis of Hong Kong oysters. Background technique [0002] Hong Kong oyster (Crassostrea hongkongensis) is the main economic species cultured in the coastal areas of South my country. It likes to live near estuaries or places with fresh water injection. It is mainly distributed in Guangdong, Guangxi and other places, with an annual output of more than 1.7 million tons and an output value of 30 billion. Yuan (China Fisheries Yearbook, 2019). Compared with other types of oysters, the value of Hong Kong oysters is mainly reflected in their large body, fat meat, and delicious taste. In addition, their market value is much higher than that of other types of oysters, and they are favored by consumers in Guangdong, Guangxi, Hong Kong, Macao, and Southeast Asian countries (Zhang Yuehuan, etc....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 肖述喻子牛张扬向志明
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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