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Method for Inducing Pengze Crucian Carp Gynogenetic Development by Culperus chinensis

A technology of gynogenesis and Pengze crucian carp, which is applied in the field of gynogenesis of Pengze crucian carp inducing the gynogenesis of Pengze crucian carp, to achieve the effect of strong operability and good adaptability

Active Publication Date: 2021-02-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Pengze crucian carp and the Culperus chinensis belong to different subfamilies of fish, and there are great differences between them in terms of biological characteristics such as genetics, taxonomy, and body shape. So far, there has been no Culprit persicae at home and abroad to induce the gynogenesis of the Pengze crucian carp. Therefore, it is of great application value to explore a method to induce the gynogenesis of Pengze crucian carp and to study and analyze the biological characteristics of its offspring.

Method used

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  • Method for Inducing Pengze Crucian Carp Gynogenetic Development by Culperus chinensis
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  • Method for Inducing Pengze Crucian Carp Gynogenetic Development by Culperus chinensis

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Effect test

Embodiment 1

[0032] The method for inducing Pengze crucian crucian carp gynogenetic development by crocodile cichlid comprises the following steps:

[0033] 1) The male parent of the mature cyprinus came from the Danjiangkou Reservoir, and the collection size was 2kg / tail; the female parent of the Pengze crucian carp came from the sexually mature Pengze crucian carp provided by the Jiangxi Fisheries Research Institute, and the parents were strengthened before birth.

[0034] 2) Artificial oxytocin of the female parents of Pengze crucian carp: In the middle of May, the female parents of Pengze crucian carp were injected with oxytocin drugs by two injections, and the first injection of luteinizing hormone-releasing hormone analogue (LRH-A 2 ), the second injection of the compound drug, the time interval between the two injections is 8-10 hours, and the injected Pengze crucian carp is placed in the oxytocin pool; wherein, in the first injection, the injection volume of the luteinizing hormone ...

Embodiment 2

[0047] The method for inducing Pengze crucian crucian carp gynogenetic development by crocodile cichlid comprises the following steps:

[0048] 1) The male parent of the mature cyprinus came from the Danjiangkou Reservoir, and the collection size was 2kg / tail; the female parent of the Pengze crucian carp came from the sexually mature Pengze crucian carp provided by the Jiangxi Fisheries Research Institute, and the parents were strengthened before birth.

[0049] 2) Artificial oxytocin of the female parents of Pengze crucian carp: In the middle of May, the female parents of Pengze crucian carp were injected with oxytocin drugs by two injections, and the first injection of luteinizing hormone-releasing hormone analogue (LRH-A 2 ), the second injection of the compound drug, the time interval between the two injections is 8-10 hours, and the injected Pengze crucian carp is placed in the oxytocin pool; wherein, in the first injection, the injection volume of the luteinizing hormone ...

Embodiment 3

[0056] The method for inducing Pengze crucian crucian carp gynogenetic development by crocodile cichlid comprises the following steps:

[0057] 1) The male parent of the mature cyprinus came from the Danjiangkou Reservoir, and the collection size was 2kg / tail; the female parent of the Pengze crucian carp came from the sexually mature Pengze crucian carp provided by the Jiangxi Fisheries Research Institute, and the parents were strengthened before birth.

[0058] 2) Artificial oxytocin of the female parents of Pengze crucian carp: In the middle of May, the female parents of Pengze crucian carp were injected with oxytocin drugs by two injections, and the first injection of luteinizing hormone-releasing hormone analogue (LRH-A 2 ), the second injection of the compound drug, the time interval between the two injections is 8-10 hours, and the injected Pengze crucian carp is placed in the oxytocin pool; wherein, in the first injection, the injection volume of the luteinizing hormone ...

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Abstract

The invention discloses a method for inducing Pengze crucian crucian carp gynogenetic development by Clamella chinensis. The method sequentially selects male and female parents and strengthens cultivation of the parents, artificially induces labor of the female parent of Pengze crucian carp, artificial insemination, artificial hatching, and cultivation of gynogenetic fish fry ; Obtain the gynogenetic progeny of Pengze crucian carp induced by C. The gynogenesis method of the invention has strong operability and good adaptability. During the implementation process, the fertilization rate can reach 97.20%, the hatching rate can reach 84.17%, and the fry survival rate can reach 86.32%. The morphological traits of the progeny obtained from the gynogenesis of Pengze crucian carp induced by Cichlid argyrocarp are biased towards the female parent, and the weight growth rate of the gynogenetic progeny of Pengze crucian carp induced by Cichlid carp is 33.29% faster than that of the selfed offspring of Pengze crucian carp .

Description

technical field [0001] The invention relates to the technical field of fish gynogenesis, in particular to a method for inducing the gynogenesis of Pengze crucian carp by carp cichlid. Background technique [0002] Gynogenesis is an important reproductive mode in fish parthenogenesis. The sperm required for it are provided by males with close relationship between hermaphroditism. After the sperm enters the egg, it does not fuse with the egg, but only induces the development of the egg. Is a form of apomixis or pseudomigration [1] . Gynogenesis has a very broad application prospect in fish genetic breeding and aquaculture, mainly in the rapid establishment of pure lines, gene-centromere mapping, discrimination of sex determination mechanism and utilization of unisexual populations, etc. [2] . [0003] From Hubbs et al. [3] It was discovered that the medaka fish (Poecilia formosa) in the American Amazon River is a naturally occurring gynogenetic population, and people have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 王卫民周淼
Owner HUAZHONG AGRI UNIV
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