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Gynogenesis method for carassius auratus var. pengsenensis thelykaryon induced by culter alburnus basilewsky

A technology of gynogenesis and Pengze crucian carp, which is applied in the field of gynogenesis of Pengze crucian carp inducing the gynogenesis of Pengze crucian carp, to achieve the effect of strong operability and good adaptability

Active Publication Date: 2020-05-22
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Pengze crucian carp and the Culperus chinensis belong to different subfamilies of fish, and there are great differences between them in terms of biological characteristics such as genetics, taxonomy, and body shape. So far, there has been no Culprit persicae at home and abroad to induce the gynogenesis of the Pengze crucian carp. Therefore, it is of great application value to explore a method to induce the gynogenesis of Pengze crucian carp and to study and analyze the biological characteristics of its offspring.

Method used

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  • Gynogenesis method for carassius auratus var. pengsenensis thelykaryon induced by culter alburnus basilewsky
  • Gynogenesis method for carassius auratus var. pengsenensis thelykaryon induced by culter alburnus basilewsky
  • Gynogenesis method for carassius auratus var. pengsenensis thelykaryon induced by culter alburnus basilewsky

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for inducing Pengze crucian crucian carp gynogenetic development by crocodile cichlid comprises the following steps:

[0033] 1) The male parent of the mature cyprinus came from the Danjiangkou Reservoir, and the collection size was 2kg / tail; the female parent of the Pengze crucian carp came from the sexually mature Pengze crucian carp provided by the Jiangxi Fisheries Research Institute, and the parents were strengthened before birth.

[0034] 2) Artificial oxytocin of the female parents of Pengze crucian carp: In the middle of May, the female parents of Pengze crucian carp were injected with oxytocin drugs by two injections, and the first injection of luteinizing hormone-releasing hormone analogue (LRH-A 2 ), the second injection of the compound drug, the time interval between the two injections is 8-10 hours, and the injected Pengze crucian carp is placed in the oxytocin pool; wherein, in the first injection, the injection volume of the luteinizing hormone ...

Embodiment 2

[0047] The method for inducing Pengze crucian crucian carp gynogenetic development by crocodile cichlid comprises the following steps:

[0048] 1) The male parent of the mature cyprinus came from the Danjiangkou Reservoir, and the collection size was 2kg / tail; the female parent of the Pengze crucian carp came from the sexually mature Pengze crucian carp provided by the Jiangxi Fisheries Research Institute, and the parents were strengthened before birth.

[0049] 2) Artificial oxytocin of the female parents of Pengze crucian carp: In the middle of May, the female parents of Pengze crucian carp were injected with oxytocin drugs by two injections, and the first injection of luteinizing hormone-releasing hormone analogue (LRH-A 2 ), the second injection of the compound drug, the time interval between the two injections is 8-10 hours, and the injected Pengze crucian carp is placed in the oxytocin pool; wherein, in the first injection, the injection volume of the luteinizing hormone ...

Embodiment 3

[0056] The method for inducing Pengze crucian crucian carp gynogenetic development by crocodile cichlid comprises the following steps:

[0057] 1) The male parent of the mature cyprinus came from the Danjiangkou Reservoir, and the collection size was 2kg / tail; the female parent of the Pengze crucian carp came from the sexually mature Pengze crucian carp provided by the Jiangxi Fisheries Research Institute, and the parents were strengthened before birth.

[0058] 2) Artificial oxytocin of the female parents of Pengze crucian carp: In the middle of May, the female parents of Pengze crucian carp were injected with oxytocin drugs by two injections, and the first injection of luteinizing hormone-releasing hormone analogue (LRH-A 2 ), the second injection of the compound drug, the time interval between the two injections is 8-10 hours, and the injected Pengze crucian carp is placed in the oxytocin pool; wherein, in the first injection, the injection volume of the luteinizing hormone ...

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Abstract

The invention discloses a gynogenesis method for carassius auratus var. pengsenensis thelykaryon induced by culter alburnus basilewsky. The method comprises the following steps of screening male and female parents, carrying out parent strengthening cultivation, carrying out the artificial spawning, the artificial insemination and the artificial incubation of a carassius auratus var. pengsenensis female parent, and carrying out gynogenesis fry seed cultivation in sequence to obtain a gynogenesis offspring of the carassius auratus var. pengsenensis thelykaryon induced by the culter alburnus basilewsky. The gynogenesis method disclosed by the invention has high maneuverability and good adaptability, in an implantation process, a fertility rate can be 97.20%, a hatching rate can be 84.17%, anda fry survival rate can be 86.32%. The morphology characters of the offspring obtained by the gynogenesis of the carassius auratus var. pengsenensis thelykaryon induced by the culter alburnus basilewsky is partial to a female parent, and in addition, the weight growth rate of the offspring population of the carassius auratus var. pengsenensis thelykaryon induced by the culter alburnus basilewskyis higher than the weight growth rate of a carassius auratus var. pengsenensis thelykaryon self-bred progeny population by 33.29%.

Description

technical field [0001] The invention relates to the technical field of fish gynogenesis, in particular to a method for inducing the gynogenesis of Pengze crucian carp by carp cichlid. Background technique [0002] Gynogenesis is an important reproductive mode in fish parthenogenesis. The sperm required for it are provided by males with close relationship between hermaphroditism. After the sperm enters the egg, it does not fuse with the egg, but only induces the development of the egg. Is a form of apomixis or pseudomigration [1] . Gynogenesis has a very broad application prospect in fish genetic breeding and aquaculture, mainly in the rapid establishment of pure lines, gene-centromere mapping, discrimination of sex determination mechanism and utilization of unisexual populations, etc. [2] . [0003] From Hubbs et al. [3] It was discovered that the medaka fish (Poecilia formosa) in the American Amazon River is a naturally occurring gynogenetic population, and people have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 王卫民周淼
Owner HUAZHONG AGRI UNIV
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