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Production method of spider-fibroin-implanted silk and spider-fibroin-implanted silk produced thereby

A production method and technology for spider silk, which are applied to the spider silk production method and the field of spider silk produced therefrom, can solve the problems of limited elastic properties, difficulty in artificial feeding, and insufficient strength, and achieve low cost, easy acquisition, and simple operation. Effect

Inactive Publication Date: 2020-05-26
张宏亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Spiders have the same silk-spinning function as silkworms, but unlike silkworms, spiders eat insects for a living, and their bodies are highly toxic. People will die after being infected with spider venom. Artificial breeding is very difficult, resulting in scarcity of spider silk, while silkworms are gentle in nature. , non-toxic in the body, as long as there are mulberry trees, silkworms can be raised to collect cocoons and spin silk, but the disadvantages of domestic silkworm silk are that the strength is not high enough and the elasticity is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] With the silkworm as the host, wash the viscera of the live garden spider with normal saline under the microscope under the aseptic condition indoors to prevent the dirt from contaminating the spidroin mucus in the gland duct of the spider, and insert it into the tail of the spider body with a medical needle Extract the spidroin mucus from the gland ducts inside and quickly implant it into the silkworm eggs, put them in a temperature-controlled 15°C incubator to catalyze the seeds, and wait for the silkworm eggs to hatch into young silkworms, which will be a new hybrid silkworm variety, which is called spider silkworm. .

[0050] The spider silkworm implanted with spidroin mucus feeds on mulberry leaves, and its feeding properties are the same as those of silkworms. After hybridization, the spider silkworms can live in five stages, that is, five times of peeling. They are called fifth-instar silkworms. Spider silkworms After maturity, it enters the cocooning stage and o...

Embodiment 2

[0053] With the silkworm as the host, wash the viscera of the live garden spider with normal saline under the microscope under the aseptic condition indoors to prevent the dirt from contaminating the spidroin mucus in the gland duct of the spider, and insert it into the tail of the spider body with a medical needle Extract the spidroin mucus from the gland ducts inside and quickly implant it into the silkworm eggs, put them in a temperature-controlled 18°C ​​incubator to stimulate the seeds, and wait for the silkworm eggs to hatch into young silkworms to become a new hybrid silkworm variety, called spider silkworm .

[0054] The spider silkworm implanted with spidroin mucus feeds on mulberry leaves, and its feeding properties are the same as those of silkworms. After hybridization, the spider silkworms can live in five stages, that is, five times of peeling. They are called fifth-instar silkworms. Spider silkworms After maturity, it enters the cocooning stage and obtains spide...

Embodiment 3

[0057] With the silkworm as the host, wash the viscera of the live garden spider with normal saline under the microscope under the aseptic condition indoors to prevent the dirt from contaminating the spidroin mucus in the gland duct of the spider, and insert it into the tail of the spider body with a medical needle Extract the spidroin mucus from the gland ducts inside and quickly implant it into silkworm eggs, put them in a temperature-controlled incubator at 20°C to catalyze the seeds, and wait for the silkworm eggs to hatch into young silkworms to become a new hybrid silkworm variety, called spider silkworm .

[0058] The spider silkworm implanted with spidroin mucus feeds on mulberry leaves, and its feeding properties are the same as those of silkworms. After hybridization, the spider silkworms can live in five stages, that is, five times of peeling. They are called fifth-instar silkworms. Spider silkworms After maturity, it enters the cocooning stage and obtains spider si...

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Abstract

The invention provides a production method of spider-fibroin-implanted silk. The production method of the spider-fibroin-implanted silk comprises the following step: (1) implanting silkworm eggs withspider fibroin mucus from glandular ducts of spiders in vivo so as to obtain spider-fibroin-implanted silkworm eggs; (2) hatching the silkworm eggs so as to obtain young silkworms, namely spider-fibroin-implanted silkworms; (3) feeding the spider-fibroin-implanted silkworms by adopting a silkworm feeding method; and (4) waiting for the spider-fibroin-implanted silkworm to spin silk, thereby obtaining the spider-fibroin-implanted silk. Preferably, before the step (1), the production method of the spider-fibroin-implanted silk further comprises a step (11) of rinsing internal organs of the spiders with normal saline in vivo under a microscope under sterile indoor conditions; and, between the steps (1) and the step (2), the production method of the spider-fibroin-implanted silk still furthercomprises a step (12) of putting the silkworms into a thermal container so as to accelerate spinning of the silk. The invention further provides the spider-fibroin-implanted silk produced by the production method. The production method of the spider-fibroin-implanted silk is capable of easily producing the spider-fibroin-implanted silk; and moreover, the produced spider-fibroin-implanted silk hashigher strength and elasticity compared with common silk, so that needs of new materials for military protection and medical use are met. In addition, the production method of the spider-fibroin-implanted silk is smartly designed, easy to operate, low in cost, and suitable for large-scale application and application.

Description

technical field [0001] The present invention relates to the technical field of biotechnology, more specifically, to the technical field of spider silk production, in particular to a method for producing spider silk and the spider silk produced therefrom. Background technique [0002] In order to catch food, spiders spit out spider silk to weave silk webs. Among the spiders that can weave webs, the most valuable ones are garden spiders. The spider silks produced by garden spiders mainly include drag silk and drag silk, and drag silk is mainly used to form spider webs. The drag wire and wheel-shaped web surface, the drag wire is used to adhere to the insects and provide strong elasticity when the insects struggle to prevent the insects from falling off the web. Spiders have many glandular tubes for storing spider silk, and the drag silk is made of whip It is secreted by the thyroid gland, and it mainly acts on the axis of the helical silk on the circular web. Although it is no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033D01B7/00
CPCA01K67/033D01B7/00
Inventor 祁曼华张琦
Owner 张宏亮
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