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The invention also discloses application of type 6 collagen in preparation of related drugs and grafts capable of improving regeneration nerve orderliness

A collagen and orderly technology, applied in the field of medicine, can solve the problems of loss of clustering characteristics, high axonal mismatch rate, counteracting and promoting nerve regeneration, etc., and achieve the effect of a wide range of protein sources.

Pending Publication Date: 2020-06-02
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, studies have shown that although the above methods for promoting nerve regeneration can increase the number and speed of axon regeneration, the axon growth in the graft is scattered and disorderly, and the original bundle characteristics are lost, resulting in a high axon mismatch rate[ 5]
This outcome may negate some of the neuroregenerative benefits

Method used

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  • The invention also discloses application of type 6 collagen in preparation of related drugs and grafts capable of improving regeneration nerve orderliness
  • The invention also discloses application of type 6 collagen in preparation of related drugs and grafts capable of improving regeneration nerve orderliness
  • The invention also discloses application of type 6 collagen in preparation of related drugs and grafts capable of improving regeneration nerve orderliness

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment one: Type VI collagen purification:

[0026] Amniotic membranes were isolated from fresh human placenta and washed several times with 0.5M sodium acetate to remove serum proteins. The washed amnion was minced and homogenized. Each 80 g (wet weight) of amniotic tissue was treated with 8 mg of pepsin and 0.5 M formic acid (pH 2.1) for 24 hours, and the whole process was carried out at 4 degrees Celsius. After centrifugation, the supernatant was collected, and a NaCl solution having a final concentration of 1.2 M was added to the solution to collect the collagen component as a precipitate. The pellet was redissolved in 0.05m Tris-HCl buffer (pH 8.0, containing 1M NaCl), and dialyzed against 0.5M acetic acid solution containing 1.2M NaCl. The precipitate was removed and the supernatant was further dialyzed against 1.7M NaCl in 0.5M acetic acid. The resulting pellet was resuspended in a solution containing 0.2% SDS, 1 mM EDTA, 50 mM Tris-HCl, 100 mM NaCl, pH 7....

Embodiment 2

[0029] It is basically the same as Example 1, except that type VI collagen complexes are isolated from fresh rat placenta.

Embodiment 3

[0031] It is basically the same as in Example 1, except that type VI collagen complexes are isolated from fresh mouse placenta.

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Abstract

The invention provides application of type 6 collagen in preparation of related drugs and nerve grafts capable of improving regeneration nerve orderliness. The type 6 collagen comprises human-derivedtype 6 collagen or animal-derived homologous type 6 collagen, and the animal-derived homologous type 6 collagen comprises type 6 collagen of rat, mouse or bovine. According to the invention, the type6 collagen COL6 is used as hydrogel to be filled in a nerve conduit, so that the clustering and orderliness of regenerated nerve fibers can be promoted, the regenerated nerve fibers are more close toa normal nerve tract structure in morphology, the mismatch probability of nerves and target organs is reduced, the nerve repair effect is improved, and the protein source is wide.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of type VI collagen in the preparation of related drugs and grafts capable of improving the orderliness of regenerative nerves. Background technique [0002] At present, autologous nerve transplantation is still the gold standard for the treatment of long-segment peripheral nerve injuries [1], but it will inevitably cause damage to the innervated areas of the donor nerves and surgical scars in the harvested areas. Therefore, there is an urgent need for suitable artificial nerve substitutes. At present, a variety of artificial nerve grafts, including nerve conduits and decellularized nerves, have been used clinically, but the repair effect is still not comparable to that of autologous nerve grafts [2-4]. Related morphological studies have revealed that artificial nerve grafts are ubiquitous The problem is that once the dense nerve fiber bundles in the nerve trunk ...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61P25/00C07K19/00
CPCA61K38/39A61P25/00C07K14/78C07K2319/00
Inventor 邹剑龙孙佳慧朱庆棠邱帅何富林
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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