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Method for detecting liquid-based fungi by using liquid-based flaking technology based on centrifugal method

A technology of preparation technology and detection method, applied in the field of liquid-based preparation and detection of liquid-based fungi, can solve the problems of long process time, cumbersome operation, and many reagents, etc., and achieve simple preparation method, solve cumbersome operation, and fluorescent effect. Good results

Pending Publication Date: 2020-06-05
江西业力医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a detection method for liquid-based fungus using a liquid-based film-making technology based on centrifugation. Solved the existing problems in the prior art, such as cumbersome operation, long process time, and many reagents needed

Method used

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  • Method for detecting liquid-based fungi by using liquid-based flaking technology based on centrifugal method
  • Method for detecting liquid-based fungi by using liquid-based flaking technology based on centrifugal method
  • Method for detecting liquid-based fungi by using liquid-based flaking technology based on centrifugal method

Examples

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Embodiment 1

[0026] A method for liquid-based fungus detection based on centrifugation-based liquid-based tablet technology, comprising the following steps: adding a special digestive solution into a sample bottle containing a fungal sample, and the volume ratio of the sample and the special digestive solution is 1:1 Put the sample bottle with an inverted cone-shaped bottom into the oscillator to vibrate for 5 minutes, let it stand for 10 minutes, with the tip facing down, fungi with a larger specific gravity will settle at the bottom of the tip, shake for 5 minutes, and insert a disposable pipette into the bottom of the sample bottle. Aspirate 1mL of the specimen and add it to the film-making chamber, which is installed on the film-making rack equipped with glass slides, hang the film-making rack into the centrifugal liquid-based cell slicer, centrifuge at 800r / min for 3min, and prepare into liquid-based flakes. After the liquid-based thin section is prepared, it is fixed, stained, sealed...

Embodiment 2

[0032] A method for liquid-based fungus detection based on centrifugation-based liquid-based tablet technology, comprising the following steps: adding a special digestive solution into a sample bottle containing a fungal sample, and the volume ratio of the sample and the special digestive solution is 1:1 Put the sample bottle with an inverted cone-shaped bottom into the oscillator for 10 minutes, let it stand for 30 minutes, with the tip facing down, and the fungus with a larger specific gravity will settle at the bottom of the tip, shake for 10 minutes, and insert a disposable pipette into the bottom of the sample bottle. Aspirate 2mL of the specimen and add it to the film-making chamber, which is installed on the film-making rack equipped with glass slides, hang the film-making rack into the centrifugal liquid-based cell slicer, centrifuge at 1000r / min for 5min, and prepare into a liquid-based thin section; after the liquid-based thin section is prepared, it is fixed, stained...

Embodiment 3

[0038]A method for liquid-based fungus detection based on centrifugation-based liquid-based tablet technology, comprising the following steps: adding a special digestive solution into a sample bottle containing a fungal sample, and the volume ratio of the sample and the special digestive solution is 1:1 Put the sample bottle with an inverted cone-shaped bottom into the oscillator for 6 minutes, let it stand for 15 minutes, with the tip facing down, fungi with a larger specific gravity will settle at the bottom of the tip, shake for 6 minutes, and insert a disposable pipette into the bottom of the sample bottle. Aspirate 1mL of the specimen and add it to the film-making chamber, which is installed on the film-making rack equipped with glass slides, hang the film-making rack into the centrifugal liquid-based cell slicer, centrifuge at 850r / min for 4min, and prepare into a liquid-based thin section; after the liquid-based thin section is prepared, it is fixed, stained, sealed, and...

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Abstract

The invention provides a method for detecting liquid-based fungi by a liquid-based flaking technology based on a centrifugal method. The liquid-based flaking technology based on the centrifugal methodcomprises the following steps: adding special digestive juice into a specimen bottle of a specimen containing fungi; putting the specimen bottle with the inverted-cone-shaped bottle bottom into an oscillator to oscillate; standing, precipitating fungi with downward tips and large specific gravity at the bottoms of the tips, oscillating, inserting a disposable transfer pipette into the bottom of the specimen bottle, sucking 1-2mL of specimens, adding the specimens into a slide preparation bin, mounting the slide preparation bin on a slide preparation rack filled with a glass slide, hanging theslide preparation rack into a centrifugal liquid-based cell slide preparation machine, centrifuging, and preparing a liquid-based sheet. The preparation method is simple, the prepared fungus slice isclear and observable, accurate detection of fungi is achieved, and the problems that in the prior art, operation is complex, time consumed in the process is long and a large number of reagents are needed are effectively solved.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a method for detecting liquid-based fungi using liquid-based sheet-making technology based on centrifugation. Background technique [0002] Fungi belong to eukaryotes, without plastids, and their nutritional mode is absorption without phagocytosis. The cell wall contains chitin and β-glucan. It can cause human and animal diseases, called fungal diseases, and can also cause plant diseases, human allergic diseases and mycotoxin poisoning. Fungal invasion of the human body is often secondary to other diseases, such as chronic wasting diseases such as malignant tumors and diabetes; large doses of X-ray irradiation; immunosuppressants, etc. lead to low immune function and resistance; The reaction is mostly suppressed, and it is also easy to be infected with fungi; in addition, organ transplantation, catheter intubation, radiotherapy and other applications cause local tissue ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N21/64
CPCG01N1/2813G01N21/6486G01N21/6428G01N2001/2846G01N2021/6439Y02A50/30
Inventor 张沿熊文芳吕正和陈永洪杜厚明杨平平李娟陈荣平
Owner 江西业力医疗器械有限公司
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