Desferoxamine slow-release microbubble modified composite pore electrospun scaffold and preparation method thereof

An electrospinning and deferrioxamine technology, which is applied in the field of composite pore size electrospinning scaffolds modified by deferrioxamine sustained-release microbubbles and their preparation, can solve the problems of few related research reports on the release of activators, and achieve improved release. Effects of Features

Active Publication Date: 2021-01-08
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pore expansion method usually only achieves the purpose of pore expansion, and there are few relevant research reports on the release of activating factors.

Method used

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  • Desferoxamine slow-release microbubble modified composite pore electrospun scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Preparation of deferoxamine sustained-release microbubbles

[0029] Add 8g lactide, 2g glycolide, 1g polyethylene glycol 2000 to the polymerization tube, then add 0.5g of 0.3% stannous octoate in dichloromethane solution as a catalyst, and heat the polymerization tube to 150°C under vacuum to mix The material is melted and mixed uniformly, and kept for 8h. After natural cooling, it was dissolved in acetone, precipitated and washed with water to obtain a polymer as a shell material. The hydrophilicity test contact angle of the polymer prepared above was 30°, the viscosity was 1.2, and the elongation at break was 4.5%.

[0030] Dissolve 0.5 g of deferoxamine in 10 mL of water as the inner water phase, then dissolve 5 g of the polymer in 100 mL of ethyl acetate as the oil phase, and disperse the inner water phase in the oil phase by fully stirring to obtain a W / O type emulsion; 10g of PVA and 25g of NaCl were dissolved in 500mL of water as the outer water phase, and t...

Embodiment 2

[0036] 1) Preparation of deferoxamine sustained-release microbubbles: This procedure is the same as that in Example 1.

[0037] 2) Preparation of Deferrioxamine Sustained-release Microbubbles Modified Electrospinning Scaffolds

[0038] The core solution of electrospinning is composed of deferoxamine sustained-release microbubbles, type I collagen, and hexafluoroisopropanol, the mass content of deferoxamine sustained-release microbubbles is 10%, and the mass content of type I collagen is 5%; electrospinning The shell liquid of the silk is composed of hydroxyapatite, type I collagen and hexafluoroisopropanol, the mass content of hydroxyapatite is 15%, and the mass content of type I collagen is 15%; prepared by coaxial electrospinning method, wherein , the electrostatic high voltage was 50 kV, the receiving distance was 200 mm, and the electrospinning rate was 0.05 ml / min, to prepare a deferoxamine sustained-release microbubble modified electrospinning scaffold.

[0039] 3) Prep...

Embodiment 3

[0042] 1) Preparation of deferoxamine sustained-release microbubbles: This procedure is the same as that in Example 1.

[0043] 2) Preparation of Deferrioxamine Sustained-release Microbubbles Modified Electrospinning Scaffolds

[0044] The core solution of electrospinning is composed of deferoxamine sustained-release microbubbles, type I collagen and hexafluoroisopropanol, the mass content of deferoxamine sustained-release microbubbles is 1%, and the mass content of type I collagen is 15%; electrospinning The shell liquid of the silk is composed of hydroxyapatite, type I collagen and hexafluoroisopropanol, the mass content of hydroxyapatite is 5%, and the mass content of type I collagen is 5%; prepared by coaxial electrospinning method, wherein , the electrostatic high voltage was 1 kV, the receiving distance was 50 mm, and the electrospinning rate was 0.2 ml / min, to prepare a deferoxamine sustained-release microbubble modified electrospinning scaffold.

[0045] 3) Preparatio...

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Abstract

The invention relates to a composite-aperture electrostatic spinning bracket modified with slow-release desferrioxamine microbubbles and a preparation method of the composite-aperture electrostatic spinning bracket. Glycolide and / or lactide are / is adopted as monomers for a polymerization reaction to prepare a polymer, the polymer is taken as a shell material, desferrioxamine is used as a core material, and the slow-release desferrioxamine microbubbles with the core-shell structure are prepared with a rapid emulsification method; a core liquid containing the slow-release desferrioxamine microbubbles and a shell liquid are subjected to coaxial electrostatic spinning, and an electrostatic spinning bracket modified with the slow-release desferrioxamine microbubbles is obtained; the electrostatic spinning bracket is irradiated by laser for reaming, and the composite-aperture electrostatic spinning bracket modified with the slow-release desferrioxamine microbubbles is obtained. The bionic bracket with 10-500 micrometer composite aperture is obtained, good conditions are provided for growth of new bone and blood vessels, furthermore, release of the desferrioxamine is realized, sufficientconcentration can be obtained at an early stage, and a long-term slow-release effect can be kept.

Description

technical field [0001] The present application relates to a tissue scaffold for bone repair and a preparation method thereof, in particular to a composite aperture electrospinning scaffold modified by deferoxamine sustained-release microbubbles and a preparation method thereof. Background technique [0002] The repair of critical bone defects is a difficult clinical problem, and the current gold standard for the treatment of critical size bone defects is autologous bone transplantation. However, the amount of bone tissue in the donor site is limited, and various complications such as bone defect, pain, and infection in the donor site after surgery severely limit the popularization and application of autologous bone transplantation. In the repair of critical size bone defects, insufficient vascularization of grafts can easily lead to sequestrum formation and difficult recovery of limb function. Therefore, the development of grafts with good vascularization and functionalizati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/18A61L27/50D01D5/00D01D5/34
CPCA61L27/18A61L27/50A61L27/54A61L2300/204A61L2300/412A61L2300/602A61L2300/622A61L2430/02D01D5/0015D01D5/34C08L67/04
Inventor 崔翔刘建恒李明刘鐘阳孙国飞张里程唐佩福
Owner GENERAL HOSPITAL OF PLA
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