Method for establishing fingerprint spectrum of rhizoma alismatis decoction and fingerprint spectrum

A technology for fingerprinting and establishing methods, applied in the field of quality identification of traditional Chinese medicine, to achieve the effects of high precision, good reproducibility, accurate quality detection and evaluation

Inactive Publication Date: 2020-07-10
SHAANXI INST OF INT TRADE & COMMERCE
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AI Technical Summary

Problems solved by technology

But there is no report on the establishment of Alisma Decoction fingerprint by UPLC technology, so the present invention adopts UPLC technology to establish the fingerprint of Alisma Decoction to provide a fast and effective method for the quality control of Alisma Decoction

Method used

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  • Method for establishing fingerprint spectrum of rhizoma alismatis decoction and fingerprint spectrum
  • Method for establishing fingerprint spectrum of rhizoma alismatis decoction and fingerprint spectrum
  • Method for establishing fingerprint spectrum of rhizoma alismatis decoction and fingerprint spectrum

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Effect test

preparation example Construction

[0067] 4. Preparation of the test solution

[0068] The experiment investigated different extraction solvents, and the specific operation was as follows: take 5 mL of decoction concentrate of Alisma decoction Z1 sample, and precisely add extraction solvents (20% methanol, 40% methanol, 60% methanol, 80% methanol, 100% methanol , 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol, 95% ethanol) 25ml, ultrasonically extract 30min, let cool, centrifuge, get the supernatant liquid injection analysis. The results showed that 80% v / v methanol had the best extraction effect, and the peak shape and separation were better. The results are shown in Table 3.

[0069] Table 3 The main peak areas of the fingerprints obtained by different extraction solvents

[0070]

[0071] According to the above-mentioned investigation results, the preparation method of the test solution is determined as follows: take Alisma decoction decoction concentrated solution 5mL, accurately weighed, accuratel...

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Abstract

The invention discloses a method for establishing a fingerprint spectrum of rhizoma alismatis decoction and the fingerprint spectrum. The method comprises the following steps: (1) preparing rhizoma alismatis decoction by adopting rhizoma alismatis and bighead atractylodes rhizomes with different producing areas and different batch numbers, and extracting rhizoma alismatis water decoction by utilizing a methanol solution to obtain a test solution; dissolving atractylodes macrocephala lactone I, atractylodes macrocephala lactone II, atractylodes macrocephala lactone III, alisol A, alisol B and 23-acetyl alisol B by using a methanol solution to prepare a reference substance solution; and (2), carrying out ultra-high liquid chromatography analysis on the test solution and the reference solution, and recording the chromatogram. The rhizoma alismatis decoction fingerprint spectrum established by the method provided by the invention can effectively characterize the quality of the rhizoma alismatis decoction, and provides a basis for monitoring the quality of medicinal materials. Meanwhile, the method has the advantages of being simple, rapid, stable, good in reproducibility, high in precision and the like.

Description

technical field [0001] The invention belongs to the technical field of quality identification of traditional Chinese medicines, in particular to a method for establishing fingerprints of Alisma decoction and the fingerprints of Alisma decoction established by the method. Background technique [0002] Alisma Decoction comes from the "Golden Chamber Synopsis" by Zhang Zhongjing in the Eastern Han Dynasty. It is composed of Alisma and Atractylodes Rhizome. It is sweet in taste and cold in nature and returns to the kidney and bladder meridian; stomach meridian. Alisma decoction has the effect of eliminating water, regulating kidney and spleen. It was used in ancient times to treat fainting and vertigo. At present, it is widely used in the treatment of vertigo diseases such as Meniere's disease, benign paroxysmal positional vertigo, and vertebrobasilar insufficiency. Although the composition is simple, but the curative effect is remarkable, and it is a variety in the "Ancient Cl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/04G01N30/86
CPCG01N30/02G01N30/04G01N30/8679G01N30/8686G01N2030/042
Inventor 唐文强高艳蓉刘斌仝红娟杨长花刘峰
Owner SHAANXI INST OF INT TRADE & COMMERCE
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