Method for simultaneously detecting 103 antibiotic residues in fresh eggs by ultrahigh pressure liquid chromatography-tandem mass spectrometry

Abstract

The invention discloses a method for simultaneously detecting 103 antibiotics in fresh eggs. The method mainly comprises the following steps: carrying out solution extraction on a sample, and purifying and enriching by using a Prime HLB solid-phase extraction column; separating through a reversed-phase chromatographic column, detecting in a multi-reaction monitoring mode by adopting an ultra-highperformance liquid chromatography tandem mass spectrometer, determining chromatographic peak retention time and parent ion / daughter ion pairs of various antibiotics, and then carrying out qualitativeand quantitative analysis. The method can be used for simultaneously detecting 103 antibiotics such as quinolones, sulfonamides, macrolides, lactams, tetracyclines, antifungal bacteria and nitroimidazoles in animal derived food such as fresh eggs, fresh duck eggs and fresh quail eggs. The detection method disclosed by the invention is sensitive, accurate and rapid, and 103 antibiotics can be qualitatively and quantitatively analyzed at the same time within only 18 minutes on an instrument. And the detection method provided by the invention also has a certain reference value for detection of other antibiotics.

Description

technical field [0001] The invention belongs to the field of antibiotic detection and relates to an ultrahigh pressure liquid chromatography tandem mass spectrometry method for simultaneously detecting 103 kinds of antibiotic residues in fresh eggs. Background technique [0002] Antibiotics are a type of secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) or higher animals and plants in the course of life, which have anti-pathogen or other activities, and can interfere with the development of other biological cells. Chemical substances . According to a research report published in the journal PNAS, during the 15-year period from 2000 to 2015, the global use of antibiotics surged by 65%, and most of the increase came from the increase in the use of antibiotics in low- and middle-income countries. Studies have shown that in 2000, most of the countries with the largest per capita consumption of antibiotics were developed countries or...

AI Technical Summary

Problems solved by technology

In recent years, many domestic and foreign studies on the detection of glycopeptide antibiotics by high performance liquid chromatography mass spectrometry have mostly focused on single veterinary drug residues in fresh egg samples, and there are also a few reports on multiple veterinary drug residues. Analytical studies of multi-residue compounds of single antibiotics have not been reported in

In particular, there is no report on the method for the simultaneous detection of 103 antibiotics in fresh egg samples based on the ultra-high pressure liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) platform using Prime HLB solid-phase extraction column purification

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