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Brevibacillus brevis for controlling root knot nematode and application of brevibacillus brevis

A technology of Bacillus brevis, root knot nematode, applied in the field of microbial pesticides and microbial inoculants, can solve problems such as applications that have not been reported, and achieve the effect of low cost of use

Active Publication Date: 2020-10-16
山东滇鲁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the activity of Bacillus brevus to root-knot nematode and its application in Chinese medicinal materials have not been reported, thus highlighting the functional specificity of the microorganisms used in the present invention

Method used

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  • Brevibacillus brevis for controlling root knot nematode and application of brevibacillus brevis
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  • Brevibacillus brevis for controlling root knot nematode and application of brevibacillus brevis

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Cultivation of DL01 strain and preparation of bacterial agent

[0023] 1) Cultivation of DL01 strain slant seeds in a test tube: the strain was inoculated on the slant surface of LB medium, cultured at 28° C. for 1 day to obtain slant seeds.

[0024] 2) Liquid seed culture of the DL01 strain: inoculate the slant seeds into a conical flask containing beef extract peptone liquid medium, culture on a shaker at 28° C. and 150-180 rpm for 2 days to obtain liquid seeds.

[0025] 3) Large-scale cultivation of DL01 strain fermenter: the liquid seeds are inserted into the beef extract peptone liquid medium in the fermenter at a ratio of 3% (V / V), and the culture conditions in the 1000 liter fermenter are controlled at: temperature 32 ℃, stirring speed 150rpm, fermentation time 72 hours.

[0026] 4) Preparation of DL01 microbial agent: the microbial cells and their metabolites obtained through fermenter cultivation are mixed with an appropriate amount of diatomaceous earth, drie...

Embodiment 2

[0028] Cultivation of DL01 strain and preparation of bacterial agent

[0029] 1) Cultivation of DL01 strain slant seeds in a test tube: the strain was inoculated on the slant surface of LB medium, and the culture condition was 30° C. for 2 days to obtain slant seeds.

[0030] 2) Liquid seed culture of the DL01 strain: Inoculate the slanted seeds into a triangular flask filled with beef extract-peptone liquid medium, and cultivate them at 30° C. for 2 days to obtain liquid seeds.

[0031] 3) Large-scale cultivation of DL01 strain fermenter: the liquid seeds are inserted into the beef extract peptone liquid medium in the fermenter at a ratio of 3% (V / V), and the culture conditions in the 1000 liter fermenter are controlled at: temperature 32 ℃, stirring speed 150rpm, fermentation time 72 hours.

[0032] 4) Preparation of DL01 microbial agent: the microbial cells and their metabolites obtained through fermenter cultivation are mixed with an appropriate amount of diatomaceous ear...

Embodiment 3

[0034] Cultivation of DL01 strain and preparation of bacterial agent

[0035] 1) DL01 strain test tube slant seed culture: the strain was inoculated on the LB medium slant, cultivated at 32°C for 3 days to obtain slant seeds;

[0036] 2) Liquid seed culture of the DL01 strain: inoculate the slant seeds into a triangular flask containing beef extract peptone liquid medium, culture on a shaker at 32°C and 150-180 rpm for 3 days to obtain liquid seeds;

[0037] 3) Large-scale cultivation of the DL01 strain fermenter: the liquid seeds were inserted into the beef extract peptone liquid medium in the 1000 liter fermenter at a ratio of 3% by volume, and the culture conditions were controlled at: temperature 32 ° C, stirring speed 150 rpm, fermentation Time 72 hours.

[0038] 4) Preparation of DL01 microbial agent: mix the microbial cells and their metabolites obtained by fermenter cultivation with 2 times the volume of diatomaceous earth, dry below 65°C or air-dry until the moisture...

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Abstract

The invention relates to brevibacillus brevis and application thereof, and belongs to the technical field of microbial pesticides and microbial fertilizers. A strain DLO1 of brevibacillus brevis has been collected in China General Microbiological Culture Collection Center on June 16, 2020; the address is No.3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; the collection number is CGMCC No.20096. Microbial cells of brevibacillus brevis and metabolites of the microbial cells can be mixed with a conventional pesticide adsorption carrier and an auxiliary agent to prepare a DL01 bacterial agent. The invention further relates to application of the DL01 strain to preparation of the bacterial agent for controlling the root knotnematode. The bacterial agent prepared by the DL01 strain can effectively control the root knot nematode of traditional Chinese materials, and has the characteristics of being low in cost, high in control efficiency, having no residues, and being safe to humans and animals.

Description

Technical field: [0001] The invention relates to the application of a strain of Bacillus brevus in preventing and treating root-knot nematode disease, and belongs to the technical field of microbial pesticides and microbial agents. Background technique: [0002] Crop pathogenic nematodes cause direct economic losses of up to 157 billion U.S. dollars every year in the world. Among them, root-knot nematodes are the most harmful and cause the greatest losses, and the damage and loss of traditional Chinese medicines are particularly prominent. At present, the planting area of ​​Chinese herbal medicines in my country is 667,000 hm 2 Above, there are more than 1,000 Chinese herbal medicine production areas, and the cultivation of Chinese herbal medicines has become the main source of income for farmers in many places in my country to get rid of poverty and become rich. Panax notoginseng, American ginseng and ginseng are the leading varieties of Chinese medicinal materials; in rec...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A01N63/20A01P5/00C12R1/08
CPCC12N1/20C12N1/02A01N63/20C12R2001/08C12N1/205
Inventor 莫明和刘鲁民王瑞宾陈佳清
Owner 山东滇鲁生物科技有限公司
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