Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ginseng coumarin compounds and their application in medicine

A technology of ginseng coumarin and compounds, applied in the field of medicine, can solve problems such as adverse drug reactions and expensive drugs, and achieve the effects of reducing adverse reactions, reducing costs and prices, and reducing the burden on patients

Active Publication Date: 2020-12-29
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The western medicines currently used to treat RA are very expensive, and most of them will have many adverse reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ginseng coumarin compounds and their application in medicine
  • Ginseng coumarin compounds and their application in medicine
  • Ginseng coumarin compounds and their application in medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Extraction and purification of three coumarin compounds in ginseng root

[0040] Take 10.0 kg of tree ginseng roots, heat and reflux three times with 100 kg of 70% ethanol for 3 hours each time, and concentrate the extract under reduced pressure to obtain 1.3 kg of extract. The extract was suspended in 1L of water, extracted successively with equal volumes of petroleum ether, ethyl acetate and n-butanol, and concentrated to dryness respectively. Take 216 g of n-butanol extract, mix the sample with silica gel (weight ratio 1:1), put it on a silica gel column (column size: 11*120cm), and mix it with dichloromethane-methanol (100:0→0:100, v / v) Gradient elution to obtain 9 fractions Frs. C1-C9. (1st to 9th fractions)

[0041] Dichloromethane-methanol 5:1 eluted fraction Fr.C7 (the seventh fraction 70.0 g) was subjected to octadecylsilane column chromatography (column specification: 4.2*40 cm), methanol-water (5%→30 %) gradient elution to obtain 3 fractions Fr...

Embodiment 2

[0043] Example 2: Structural identification of three coumarin compounds in ginseng

[0044]The compound of formula I, compound of formula II and compound of formula III separated in Example 1 were identified as three kinds of ginseng Soybean compounds. Wherein, the formula I structural compound is a new coumarin derivative that has not been reported in the literature, and the formula II structural compound and the formula III structural compound are known compounds (but the activity of treating rheumatoid arthritis and rheumatoid arthritis disease has not been see report).

[0045] Wherein, the compound of formula I is light yellow powder. HR-ESI-MS gives the quasi-molecular ion peak m / z 531.17083 ([M+H] + , Calculated value: 531.17066), determine its molecular formula as C 23 h 31 o 14 . The hydrogen spectrum of formula I structure compound is as figure 1 As shown, (DMSO- d 6 , 600MHZ) there are 2 methoxy hydrogen signals δ H 3.90 and 3.82 (each, 3H, s, 8 / 6-CH 3 ...

Embodiment 3

[0053] Embodiment 3: Cytotoxicity evaluation experiment of coumarin compounds in ginseng to MH7A

[0054] Cell culture: MH7A cells (Guangdong Geneo Biotechnology Co., Ltd.) were subcultured for 5-10 passages, and the culture conditions were penicillin (final concentration: 100 U / mL), streptomycin (final concentration: 100 μg / mL), 10 High-glucose DMEM medium with % FBS, when the cells are confluent to 90%, discard the old medium, wash the cells twice with 2 ml PBS, discard the PBS, add 2 mL of 0.25% (w / v) Trypsin-0.53 Mix the digestion solution with mM EDTA and observe it under a microscope for about 30 s. When the cells become round, quickly add 2 ml of complete medium to stop the digestion, and gently pipette to collect the cells. Centrifuge at 800 rpm at 4°C for 5 min, discard the supernatant, resuspend the cells with complete medium, culture in separate bottles, and change the medium every other day.

[0055] Cell viability assay: adjust MH7A cell density to 5 × 10 4 cell...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of drug development for the treatment of inflammatory diseases, in particular to the application of coumarin compounds in tree ginseng in the preparation of drugs for treating rheumatoid arthritis or rheumatoid arthritis, and the coumarin compound in tree ginseng It has one of the following chemical structural formulas: It has been confirmed by activity screening experiments that the three coumarin compounds in tree ginseng have significant effects on the release of nitric oxide from MH7A cells induced by tumor necrosis factor α (TNF-α). For inhibition, the half inhibitory concentration (IC50) values ​​were 7.87±0.7μM (Formula I), 13.82±1.1μM (Formula II) and 39.88±2.3μM (Formula III). The invention provides a new direction of drug research for the clinical development of rheumatoid arthritis and the treatment of rheumatoid arthritis.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of coumarin compounds in tree ginseng in the preparation of medicines for treating rheumatoid arthritis or rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (RA) is a common chronic, systemic autoimmune disease that can cause joint destruction, joint deformity, and even disability. The western medicines currently used to treat RA are very expensive, and most of them have many adverse reactions. Therefore, it has broad prospects to find drugs for the treatment of RA from natural medicines, especially traditional ones. [0003] MH7A cells are a kind of human rheumatoid arthritis fibroblast-like synoviocytes, which are closely related to rheumatoid arthritis and rheumatoid arthritis. MH7A cells have the activity of releasing nitric oxide, which will aggravate inflammation. Therefore, if it is found that the natural product can inhibit the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/075C07H1/08A61K31/37A61K31/7048A61P19/02A61P29/00
CPCA61K31/37A61K31/7048A61P19/02A61P29/00C07H1/08C07H17/075
Inventor 杨丽何军伟刘荣华
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com