The invention relates to the field of biology, and in particular to a method for extracting collagen from giant salamander. The method comprises the following steps: sterilization treatment, acid leaching, water washing, alkaline leaching, secondary water washing, salt leaching, enzymolysis and purifying. According to the method disclosed by the invention, the collagen is extracted by utilizing a mixture of the skin, the muscle and the bones of the giant salamander as a raw material, and the resource of the giant salamander is fully utilized; through the operation steps of sterilization treatment, acid leaching, water washing, alkaline leaching, secondary water washing, salt leaching, enzymolysis and purifying, the extracting rate and the purity of giant salamander collagen are effectively increased, determination shows that the extracting rate is up to 75.2 percent and the purity is up to 97.8 percent, the demands on the collagen and the pursuit to quality of a current market are effectively met, and the market application value of the collagen is increased.