Method for extracting amino acids from giant salamanders
A technology of amino acids and giant salamanders, applied in the biological field, can solve the problems of no relevant research reports on amino acids, and achieve the effects of improving market application value, significant bactericidal effect, and improving extraction rate
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Embodiment 1
[0029] A method for extracting amino acids from giant salamanders, comprising the following steps:
[0030] (1) Sterilization treatment: After washing the raw materials of giant salamanders with clean water, soak them in 0.012% salt water at room temperature for 30 minutes, remove the raw materials, then soak the giant salamanders in sterilizing solution for 40 minutes at 50°C, and remove the raw materials Drain and dry to obtain giant salamander powder; the giant salamander raw material is mixed with giant salamander skin and giant salamander muscle in a mass ratio of 0.1:0.8; Mix and pulverize, add water 1-2 times the mass of the pulverized material, heat and boil for 30 minutes, cool to room temperature, add ethanol in an amount 5 times the mass of the pulverized material, let stand for 120 minutes, recover ethanol, filter, and filter the filtrate Concentrate to half the volume and irradiate with ultraviolet light with a wavelength of 215nm for 8 minutes;
[0031] (2) Hydr...
Embodiment 2
[0039] A method for extracting amino acids from giant salamanders, comprising the following steps:
[0040] (1) Sterilization treatment: After washing the raw materials of giant salamanders with clean water, soak them in 0.018% salt water at room temperature for 40 minutes, remove the raw materials, and then soak the giant salamanders in sterilizing solution for 50 minutes at 60°C, and remove the raw materials Drain and dry to obtain giant salamander powder; the giant salamander raw material is mixed with giant salamander skin and giant salamander muscle in a mass ratio of 0.2:1.2; Mix and crush, add water twice the mass of the crushed material, heat and boil for 40 minutes, cool to normal temperature, add ethanol in an amount 5 times the mass of the crushed material, let stand for 160 minutes, recover ethanol, filter, and concentrate the filtrate to Half of the volume can be irradiated with ultraviolet rays with a wavelength of 225nm for 12 minutes;
[0041] (2) Hydrolysis: ...
Embodiment 3
[0049] A method for extracting amino acids from giant salamanders, comprising the following steps:
[0050] (1) Sterilization treatment: After washing the raw materials of giant salamanders with clean water, soak them in 0.015% salt water at room temperature for 35 minutes, remove the raw materials, then soak the giant salamanders in sterilizing solution for 45 minutes at 55°C, and remove the raw materials Drain and dry to obtain giant salamander powder; the giant salamander raw material is mixed with giant salamander skin and giant salamander muscle at a mass ratio of 0.15:1; Mix and pulverize, add water 1-2 times the mass of the pulverized material, heat and boil for 35 minutes, cool to room temperature, add ethanol in an amount 5 times the mass of the pulverized material, let stand for 140 min, recover ethanol, filter, and filter the filtrate Concentrate to half the volume and irradiate with ultraviolet light with a wavelength of 220nm for 10 minutes;
[0051] (2) Hydrolys...
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