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Method for extracting amino acids from giant salamanders

A technology of amino acids and giant salamanders, applied in the biological field, can solve the problems of no relevant research reports on amino acids, and achieve the effects of improving market application value, significant bactericidal effect, and improving extraction rate

Inactive Publication Date: 2018-04-13
GUIYANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Compound preparations composed of a variety of amino acids play a very important role in modern intravenous nutrition infusion and "element diet" therapy. They play an active role in maintaining the nutrition of critically ill patients and saving their lives. They have become one of the indispensable medical varieties in modern medicine. 1. Therefore, the extraction process of amino acids has attracted more and more attention; animals are rich in a large amount of amino acids, such as the application number 201410380978.5 "Method for Extracting Amino Acids from Oyster Meat", take oyster meat and cook it with high-temperature steam at 90-100°C for 5-15 minutes, After being cooked and sterilized, the weight ratio of fresh oyster meat to salt and hot water at 60-70°C is 100:3-10:78-82. Add salt and hot water to cooked oyster meat, Grind into a slurry at high temperature. When the slurry is cooled to 40°C, add 1% fresh oyster meat weight pre-expanded fermented strains, stir evenly, and seal and ferment for 30-50 days at 20-25°C. Fully stir once every 2 days, filter the fermented liquid, bottle the clarified filtrate, spray and sterilize it at 85-95°C for 20 minutes, and obtain a reddish-brown or brown oyster amino acid liquid with a delicious smell. Giant salamander is rich in Amino acids, amino acids and other substances, the extraction of amino acids from giant salamanders has been related to patent research reports, but there are no relevant research reports on the extraction of amino acids from giant salamanders

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for extracting amino acids from giant salamanders, comprising the following steps:

[0030] (1) Sterilization treatment: After washing the raw materials of giant salamanders with clean water, soak them in 0.012% salt water at room temperature for 30 minutes, remove the raw materials, then soak the giant salamanders in sterilizing solution for 40 minutes at 50°C, and remove the raw materials Drain and dry to obtain giant salamander powder; the giant salamander raw material is mixed with giant salamander skin and giant salamander muscle in a mass ratio of 0.1:0.8; Mix and pulverize, add water 1-2 times the mass of the pulverized material, heat and boil for 30 minutes, cool to room temperature, add ethanol in an amount 5 times the mass of the pulverized material, let stand for 120 minutes, recover ethanol, filter, and filter the filtrate Concentrate to half the volume and irradiate with ultraviolet light with a wavelength of 215nm for 8 minutes;

[0031] (2) Hydr...

Embodiment 2

[0039] A method for extracting amino acids from giant salamanders, comprising the following steps:

[0040] (1) Sterilization treatment: After washing the raw materials of giant salamanders with clean water, soak them in 0.018% salt water at room temperature for 40 minutes, remove the raw materials, and then soak the giant salamanders in sterilizing solution for 50 minutes at 60°C, and remove the raw materials Drain and dry to obtain giant salamander powder; the giant salamander raw material is mixed with giant salamander skin and giant salamander muscle in a mass ratio of 0.2:1.2; Mix and crush, add water twice the mass of the crushed material, heat and boil for 40 minutes, cool to normal temperature, add ethanol in an amount 5 times the mass of the crushed material, let stand for 160 minutes, recover ethanol, filter, and concentrate the filtrate to Half of the volume can be irradiated with ultraviolet rays with a wavelength of 225nm for 12 minutes;

[0041] (2) Hydrolysis: ...

Embodiment 3

[0049] A method for extracting amino acids from giant salamanders, comprising the following steps:

[0050] (1) Sterilization treatment: After washing the raw materials of giant salamanders with clean water, soak them in 0.015% salt water at room temperature for 35 minutes, remove the raw materials, then soak the giant salamanders in sterilizing solution for 45 minutes at 55°C, and remove the raw materials Drain and dry to obtain giant salamander powder; the giant salamander raw material is mixed with giant salamander skin and giant salamander muscle at a mass ratio of 0.15:1; Mix and pulverize, add water 1-2 times the mass of the pulverized material, heat and boil for 35 minutes, cool to room temperature, add ethanol in an amount 5 times the mass of the pulverized material, let stand for 140 min, recover ethanol, filter, and filter the filtrate Concentrate to half the volume and irradiate with ultraviolet light with a wavelength of 220nm for 10 minutes;

[0051] (2) Hydrolys...

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Abstract

The invention relates to the field of a biological technology, in particular to a method for extracting amino acids from giant salamanders. The method comprises the following steps of (1) performing sterilization treatment; (2) performing hydrolization; (3) performing acidolysis; (4) performing neutralizing; (5) performing acidolysis for the second time; (6) performing neutralizing for the secondtime; (7) performing extraction; and (8) performing purification. Giant salamander skin and giant salamander muscle are mixed as raw materials for extracting the amino acids, giant salamander resources are sufficiently used, and through operation steps of sterilization treatment, hydrolization, acidolysis, neutralizing, acidolysis for the second time, neutralizing for the second time, extraction and purification, the extraction rate of amino acids in the giant salamander is effectively increased. Determination proves that the extraction rate of the amino acids is 82% or above, the purity is ashigh as 97% or above, requirements for the content and the quality of the amino acids in the market at current are effectively met, and the market application value of the amino acids is increased.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for extracting amino acids from giant salamanders. Background technique [0002] Giant salamander, also known as giant salamander, belongs to Amphibian Caurus order and is known as soft gold. It is rich in protein and trace elements, as well as a small amount of fat. Among them, 8 kinds of amino acids are essential amino acids for human body. They not only have high nutritional value, but also can Prevent many diseases, improve human immunity and cell metabolism. At the same time, giant salamander has high application value in food and medicine, such as: beauty and beauty, prevention of cardiovascular diseases, anti-aging, anti-cancer, etc. It is a very good health food. In recent years, with people's understanding of the giant salamander, there are more and more researches on the giant salamander at home and abroad. In order to better utilize the nutritional value a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/175A23L17/20
CPCA23L17/20A23L33/175A23V2002/00A23V2300/14A23V2250/06A23V2250/2042A23V2250/21A23V2300/38
Inventor 曹宇李灿杨文佳孟永禄王丽娟许抗抗杨大星
Owner GUIYANG UNIV
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