Application of notoginsenoside R1

A technology of notoginseng saponins and drugs, applied in the field of medicine, can solve problems such as NTR1 that have not been shown by research

Inactive Publication Date: 2014-06-25
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there are no reports about NTR1 being able to intervene or treat Alzheimer's disease, and no studies have shown that NTR1 can inhibit the accumulation of Aβ

Method used

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  • Application of notoginsenoside R1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 10

[0061] Example 1 The effect of notoginseng saponin R1 on the results of the Morris water maze experiment

[0062] Fifty APP / PS1 transgenic AD model mice were taken, and the temperature of the feeding environment was controlled at 25±2°C and the humidity was 40-80%. Alternating light and dark for 12 hours, feeding normal sterile mouse chow, and drinking water freely. The animals were bred until they were 3 months old, and the APP / PS1 mice were randomly divided into 5 groups, with 10 mice in each group. They are: model control group, Aricept positive control group, NTR1 low-dose group, NTR1 high-dose group, and C57 normal control group. The mice in the 5 groups were intragastrically administered at the same time every day for 4 months until July. Age, conduct behavioral evaluation and detection of AD neurological damage-related indicators. The treatment doses of each group are shown in Table 1:

[0063] Table 1 Dosage of mice in each group

[0064]

[0065] The mice in ea...

Embodiment 2、 3

[0076] Example 2. Effect of notoginseng saponin R1 on the expression of ChAT and CcO and the content of malondialdehyde in the cortex of APP / PS1 transgenic AD model mice

[0077] The mice that had undergone the water maze experiment in Example 1 were taken, and the mice were killed by necking, and the brain tissue was quickly taken out to prevent autolysis. The brain tissue was washed with PBS, the cerebellum and olfactory bulb were removed, and the brain was cut into two halves along the central axis. The left hemibrain was immersed in 4% paraformaldehyde for tissue fixation; the cortex and hippocampus tissues of the right hemibrain were carefully separated, snap-frozen in liquid nitrogen, and stored in a -80°C refrigerator for subsequent biochemical analysis.

[0078] 1. Preparation of Paraffin Sections

[0079] Tissue fixation: the brain was placed in about 50 times the volume of 4% paraformaldehyde solution, replaced with a new 4% paraformaldehyde solution after half an h...

Embodiment 3、 3

[0127] Example 3. Effect of notoginseng saponin R1 on the level of IDE in the brain of APP / PS1 transgenic AD model mice

[0128] The Western bolt method was used to analyze the IDE protein content in the mouse cortex tissue frozen in Example 2 after the water maze test, and the expression of IDE mRNA in the mouse cortex was analyzed by rtPCR.

[0129] 1. Western blot of IDE protein in mouse cortex

[0130] Sample Preparation:

[0131] Take about 20 mg of the mouse cortex tissue frozen in Example 2 after the water maze test, and add 200 μL of RIPA lysate containing 1% Cocktail protease inhibitor and 1% protein phosphatase inhibitor. Homogenize in an ice bath with an electric homogenizer, and then perform ultrasonic cracking (power 21%). Centrifuge at 12,000 rpm for 10 min in a low-temperature centrifuge at 4°C, take the supernatant, measure the protein concentration with a BCA kit, and adjust the protein concentration with the lysate. Add 2×Loading Buffer (20mM Tris, pH6.8, ...

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Abstract

The invention relates to the field of medicines, and particularly relates to an application of notoginsenoside R1. Experiments prove that NTR1 can be used for improving the cognition, learning and memory capabilities of an APP / PS1 transgenic AD mouse, restoring the intracerebral ChAT level of the mouse, reducing the intracerebral malondialdehyde level, and improving the mitochondrial function of the AD mouse by improving the CcO activity. In addition, the NTR1 also can be used for increasing the IDE level so as to promote degradation of A beta and inhibit the accumulation of the A beta. Therefore, the invention further provides the application of the notoginsenoside R1 to preparation of a medicament for treating an alzheimer disease. Moreover, experiments prove that the notoginsenoside R1 causes no influences to cell viability within a concentration range of 0-500 micromoles, so the notoginsenoside R1 is proved to be safe in medication.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of notoginseng saponin R1. Background technique [0002] Panax notoginseng is the root of Panax notoginseng (Burk.) F.H.Chen, an Araliaceae plant, which has the effects of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain. Modern pharmacological studies have shown that Panax notoginseng has the effects of delaying aging, dilating blood vessels, and improving microcirculation. The main active ingredient in Panax notoginseng is Panax notoginseng saponins (Panax Noto g insen g Sa p onins, PNS), including a variety of monomeric saponins, which can promote blood circulation and improve energy metabolism. Notoginsenoside R1 (Noto g insenoside R1, NTR1), the structure is shown in formula I, [0003] [0004] It is an important component of Panax notoginseng saponins. Modern pharmacological studies have shown that NTR1 has a variety of pharm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P25/28
Inventor 张文生李智
Owner BEIJING NORMAL UNIVERSITY
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